{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:46:45Z","timestamp":1761709605551},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,6,20]],"date-time":"2019-06-20T00:00:00Z","timestamp":1560988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["8157040263"],"award-info":[{"award-number":["8157040263"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Nephrology"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Studies showed an increased visceral adipose tissue and peritoneal angiogenesis in peritoneal dialysis (PD) patients. However, the relationship between the visceral adipose expands and peritoneal angiogenesis remains unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>\n<jats:italic>Pref\u20101<\/jats:italic> (preadipocyte factor\u20101) recombinant adeno\u2010associated virus (AAV) and control AAV were constructed. Mice were divided into four groups, mice in control and PD group were injected intraperitoneally with PBS, mice in control\u2010AAV\u2010PD were injected intraperitoneally with plaque\u2010forming unit (PFU) control AAV and mice in pref\u20101\u2010AAV\u2010PD group were injected with PFU recombinant AAV. Two weeks later, control group was injected intraperitoneally with normal saline while other groups were injected intraperitoneally with 4.25% peritoneal dialysis fluid (PDF). Thirty days later, viscerall adipose tissue was collected and weighed. Pref\u20101 protein expression was measured by Western blot, and peritoneal permeability was measured by Evans blue. Cluster of differentiation 31(CD31) immunohistochemical staining was used to detect mesenteric blood vessel number, and vascular endothelial growth factor (VEGF) in serum were measured by enzyme\u2010linked immunosorbent assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Pref\u20101 protein expression increased in pref\u20101\u2010AAV\u2010PD group. Visceral adipose expanded in PD and control\u2010AAV\u2010PD group while decreased in pref\u20101\u2010AAV\u2010PD group, which approves PD fluid enhance visceral adipogensis, and the process could be inhibited by <jats:italic>Pref\u20101<\/jats:italic> recombinant AAV. The reduction of peritoneal vessel number and the decrease of vascular permeability as well as down\u2010regulation of serum vascular endothelial growth factor observed in pref\u20101\u2010AAV\u2010PD group suggested peritoneal angiogenesis could be inhibited following visceral adipose tissue reduction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Visceral adipose expands is associated with peritoneal angiogenesis in PD treatment, and prevention of visceral adipogenesis may be an alternative way to protect the validity of peritoneum. Copyright \u00a9 2019 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nep.13604","type":"journal-article","created":{"date-parts":[[2019,5,15]],"date-time":"2019-05-15T15:26:26Z","timestamp":1557933986000},"page":"248-254","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Visceral adipogenesis inhibited by Pref\u20101 is associated with peritoneal angiogenesis"],"prefix":"10.1111","volume":"25","author":[{"given":"Ran","family":"Jing","sequence":"first","affiliation":[{"name":"Department of Nephrology, Molecular Cell Lab for Kidney Disease, Renji Hospital, School of Medicine Shanghai Jiao Tong University, Shanghai Center for Peritoneal Dialysis Research  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Molecular Cell Lab for Kidney Disease, Renji Hospital, School of Medicine Shanghai Jiao Tong University, Shanghai Center for Peritoneal Dialysis Research  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Molecular Cell Lab for Kidney Disease, Renji Hospital, School of Medicine Shanghai Jiao Tong University, Shanghai Center for Peritoneal Dialysis Research  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"He","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Molecular Cell Lab for Kidney Disease, Renji Hospital, School of Medicine Shanghai Jiao Tong University, Shanghai Center for Peritoneal Dialysis Research  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Molecular Cell Lab for Kidney Disease, Renji Hospital, School of Medicine Shanghai Jiao Tong University, Shanghai Center for Peritoneal Dialysis Research  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaohui","family":"Ni","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Molecular Cell Lab for Kidney Disease, Renji Hospital, School of Medicine Shanghai Jiao Tong University, Shanghai Center for Peritoneal Dialysis Research  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiangzi","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Molecular Cell Lab for Kidney Disease, Renji Hospital, School of Medicine Shanghai Jiao Tong University, Shanghai Center for Peritoneal Dialysis Research  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,6,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.1999.00264.x"},{"key":"e_1_2_8_3_1","first-page":"238","article-title":"Evaluation of peritoneal membrane integrity","volume":"10","author":"Krediet RT","year":"1997","journal-title":"J. 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