{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:24:42Z","timestamp":1792653882306,"version":"4.5.0"},"reference-count":21,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,3,29]],"date-time":"2020-03-29T00:00:00Z","timestamp":1585440000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["Early Career Fellowship awarded to L.A.G."],"award-info":[{"award-number":["Early Career Fellowship awarded to L.A.G."]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001030","name":"National Heart Foundation of Australia","doi-asserted-by":"publisher","award":["Postdoctoral Fellowship awarded to L.A.G."],"award-info":[{"award-number":["Postdoctoral Fellowship awarded to L.A.G."]}],"id":[{"id":"10.13039\/501100001030","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Nephrology"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>The measurement of glomerular filtration rate (GFR) in experimental rodents is pivotal to understanding the progression of kidney disease and benefits of treatment strategies. A non\u2010invasive clearance device has been developed, which measures transcutaneous decay of injected FITC\u2010sinistrin in conscious rodents. The technique was validated against the well\u2010established plasma clearance method in the same mice, but on consecutive days, using only models of uninephrectomy and polycystic kidney disease. We aimed to validate this widely used technique in the same lean or obese mice, at the same time.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Five\u2010week\u2010old male C57BL\/6J mice were randomised to a high fat diet (n = 12) or normal diet (n = 11) for 10\u2009weeks. Transcutaneous and plasma clearance of FITC\u2010sinistrin were measured simultaneously in each mouse.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In lean mice, there was a positive correlation between transcutaneous and plasma derived GFR (<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.01, <jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> = .704), although there was an approximate 40% underestimation by the transcutaneous method (<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.0001). In obese mice, no correlation was observed between transcutaneous and plasma derived GFR, nor elimination half\u2010life which removes any effect of the conversion factor and injected dose. The limits of agreement in a Bland\u2010Altman plot were narrower when we used new conversion factors derived from mice in the current study and, in lean mice, a generic conversion factor which assumes 20% extracellular volume.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The non\u2010invasive clearance device may be useful for serial GFR measurements in lean and healthy mice, provided validation studies have been carried out, but its utility in obesity requires further study.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nep.13713","type":"journal-article","created":{"date-parts":[[2020,3,17]],"date-time":"2020-03-17T04:19:28Z","timestamp":1584418768000},"page":"575-581","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Validation of non\u2010invasive transcutaneous measurement for glomerular filtration rate in lean and obese <scp>C57BL<\/scp>\/<scp>6J<\/scp> mice"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4720-2577","authenticated-orcid":false,"given":"Thomas P.","family":"Mullins","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences The University of Queensland  St Lucia Australia"},{"name":"Mater Research Institute\u2010UQ Translational Research Institute  Woolloongabba Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7686-7212","authenticated-orcid":false,"given":"Wei Sheng","family":"Tan","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences The University of Queensland  St Lucia Australia"},{"name":"Duke\u2010NUS Medical School, Singapore, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4562-3888","authenticated-orcid":false,"given":"David A.","family":"Carter","sequence":"additional","affiliation":[{"name":"Institute for Molecular Bioscience The University of Queensland  St Lucia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0471-8517","authenticated-orcid":false,"given":"Linda A.","family":"Gallo","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences The University of Queensland  St Lucia Australia"},{"name":"Mater Research Institute\u2010UQ Translational Research Institute  Woolloongabba 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