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Hydrogen sulphide (H<jats:sub>2<\/jats:sub>S) exerts a protective effect in renal IRI. The present study was carried out to investigate the effects of exogenous H<jats:sub>2<\/jats:sub>S on renal IRI by regulating autophagy in mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mice were randomly assigned to control, IRI and NaHS (an H<jats:sub>2<\/jats:sub>S donor, 28, 56 and 100\u2009\u03bcmol\/kg) groups. Renal IRI was induced by clamping the bilateral renal pedicles with non\u2010traumatic arterial clamp for 45\u2009min and then reperfused for 24\u2009h. Mice were administered intraperitoneally with NaHS 20\u2009min prior to renal ischemia. Sham group mice underwent the same procedures without clamping. Serum and kidney tissues were harvested 24\u2009h after reperfusion for functional, histological, oxidative stress, and autophagic determination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with the control group, the concentrations of serum creatinine (Scr), blood urea nitrogen (BUN), and malondialdehyde (MDA), the protein levels of LC3II\/I, Beclin\u20101 and P62, as well as the number of autophagosomes were significantly increased, but the activity of superoxide dismutase (SOD) was decreased after renal IRI. NaHS pre\u2010treatment dramatically attenuated renal IRI\u2010induced renal dysfunction, histological changes, MDA concentration and p62 expression in a dose\u2010dependent manner. However, NaHS increased the SOD activity and the protein levels of LC3II\/I and Beclin\u20101.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results indicate that exogenous H<jats:sub>2<\/jats:sub>S protects the kidney from IRI through enhancement of autophagy and reduction of oxidative stress. Novel H<jats:sub>2<\/jats:sub>S donors could be developed in the treatment of renal IRI.<\/jats:p><jats:p><jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/nep14368-gra-0001-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><\/jats:sec>","DOI":"10.1111\/nep.14368","type":"journal-article","created":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T04:59:55Z","timestamp":1722315595000},"page":"645-654","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Hydrogen sulphide reduces renal ischemia\u2010reperfusion injury by enhancing autophagy and reducing oxidative 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