{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T20:21:02Z","timestamp":1760473262458,"version":"3.41.2"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T00:00:00Z","timestamp":1743120000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Nephrology"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>This research examined the role and possible regulatory mechanisms of lncRNA GAS5 in the occurrence and progression of primary nephrotic syndrome (PNS) to provide biomarkers for early screening of PNS in the clinic.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>RT\u2010qPCR was employed to assess the expression levels of GAS5 and miR\u2010144\u20105p. ROC analysis was conducted to evaluate their predictive capabilities for PNS. The interaction between GAS5 and miR\u2010144\u20105p was confirmed using a dual\u2010luciferase assay. Following this, GAS5 overexpression plasmids, along with co\u2010transfected plasmids, were introduced into podocytes to examine their impact on the inflammatory factors, oxidative stress index, cell proliferation and apoptosis. Furthermore, we performed GO and KEGG enrichment analyses, along with PPI analysis, on the target genes of miR\u2010144\u20105p to speculate on its potential functions and to identify critical genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The expression levels of GAS5 were decreased while miR\u2010144\u20105p levels were elevated in PNS patients. The diagnostic approach of serum GAS5 combined with miR\u2010144\u20105p improved the accuracy of identification. GAS5 was observed to inhibit inflammation and oxidative stress responses and the apoptosis of MPC\u20105 cells, and enhance cell proliferation. However, the overexpression of miR\u2010144\u20105p counteracted the effect of GAS5 on podocyte function. Enrichment analysis suggested the miR\u2010144\u20105p target genes could affect podocyte structure, homeostasis and cell growth. <jats:italic>PTEN<\/jats:italic> and <jats:italic>STAT3<\/jats:italic> are identified as critical regulatory targets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The sponging effect of GAS5 on miR\u2010144\u20105p caused changes in PTEN mRNA expression and could potentially prevent or mitigate PNS. GAS5 is expected to become a potential target for treating PNS.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nep.70024","type":"journal-article","created":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T04:27:08Z","timestamp":1743395228000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>Long non\u2010coding RNA Growth Arrest Specific 5<\/scp> Regulates the Podocyte Function in Nephrotic Syndrome Development via <scp>microRNA\u2010144\u20105p\/Phosphatase And Tensin Homolog<\/scp>"],"prefix":"10.1111","volume":"30","author":[{"given":"Xinyi","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases Frist Teaching Hospital of Tianjin University of Traditional Chinese Medicine  Tianjin People's Republic of China"},{"name":"National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion  Tianjin People's Republic of China"},{"name":"Graduate School Tianjin University of Traditional Chinese Medicine  Tianjin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huan","family":"Tian","sequence":"additional","affiliation":[{"name":"Clinical Laboratory Ezhou Central Hospital  Ezhou People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of the Organ Transplantation 924th Hospital of Joint Logistic Support Force of PLA  Guilin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shen\u2010ping","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of the Organ Transplantation 924th Hospital of Joint Logistic Support Force of PLA  Guilin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing\u2010sheng","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of the Organ 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