{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T01:33:26Z","timestamp":1793064806066,"version":"4.5.0"},"reference-count":26,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:00:00Z","timestamp":1792540800000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Nephrology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>\n                      Diabetic nephropathy (DN) progression involves pyroptosis. Interferon regulatory factor 7 (IRF7) can regulate pyroptosis, but its role in DN is unknown. The RNA modifier\n                      <jats:italic>N<\/jats:italic>\n                      \u2010acetyltransferase 10 (NAT10) catalyses N4\u2010acetylcytidine (ac4C) and is linked to diabetic complications. This study tested whether NAT10\u2010mediated ac4C modification of IRF7 mRNA exacerbates DN by promoting pyroptosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The NAT10 expression levels in renal tissues from DN mice and in high\u2010glucose (HG)\u2010treated HK\u20102 cells were assessed by RT\u2010qPCR and western blot. Cell viability, pyroptosis rate, levels of inflammatory cytokines (IL\u20101\u03b2, IL\u201018) and expression of pyroptosis\u2010related proteins (NLRP3, cleaved caspase\u20101, GSDMD\u2010N) were evaluated by CCK\u20108 assay, flow cytometry, ELISA and western blot, respectively. The interaction between NAT10 and IRF7 mRNA was investigated through bioinformatics prediction, RIP, acRIP\u2010qPCR and actinomycin\n                      <jats:sc>D<\/jats:sc>\n                      chase assays. In\u00a0vivo, an AAV\u2010mediated shRNA targeting NAT10 was delivered to DN mice. Renal function, histopathology, tissue inflammation and protein expression were analysed using biochemical assays, HE staining, ELISA, immunohistochemistry and western blot.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>NAT10 was upregulated in DN mice and HG\u2010exposed HK\u20102 cells. Depletion of NAT10 inhibited HG\u2010induced pyroptosis in HK\u20102 cells. Notably, NAT10 stabilized IRF7 through ac4C modification. Moreover, knockdown of NAT10 was found to alleviate HG\u2010induced HK\u20102 cells' pyroptosis and DN in mice by downregulating IRF7 expression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>NAT10 upregulation promotes DN progression by enhancing IRF7 mRNA stability through ac4C modification, which provides new therapeutic targets for DN treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/nep.70296","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:20:15Z","timestamp":1792624815000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>NAT10<\/scp>\n                    \u2010Mediated\n                    <scp>ac4C<\/scp>\n                    Modification of\n                    <scp>IRF7<\/scp>\n                    Promotes Diabetic Nephropathy Progression"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-0469-8352","authenticated-orcid":false,"given":"Jiazhong","family":"Zhang","sequence":"first","affiliation":[{"name":"Xiangyang Polytechnic University Gaotie South Road Campus  Xiangyang Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaming","family":"Li","sequence":"additional","affiliation":[{"name":"Xiangyang Polytechnic University Gaotie South Road Campus  Xiangyang Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,21]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/14791641211058856"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.11491116"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00045.2011"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2021.09.003"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2016.10.004"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2021.154748"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2014.09.018"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03464"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/gene.2011.21"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.2147\/DMSO.S514319"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes13122412"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2023.156168"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-023-01918-y"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/mco2.70015"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2023.03.023"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10565-024-09962-6"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12010-025-05416-9"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.impact.2025.100580"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1055\/a-2435-8264"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2022.04.011"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-023-04350-w"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-020-05963-w"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10142-025-01617-x"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1236923"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.13468"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41387-024-00325-y"}],"container-title":["Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nep.70296","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/nep.70296","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nep.70296","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T00:59:37Z","timestamp":1793062777000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/nep.70296"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":26,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/nep.70296"],"URL":"https:\/\/doi.org\/10.1111\/nep.70296","archive":["Portico"],"relation":{},"ISSN":["1320-5358","1440-1797"],"issn-type":[{"value":"1320-5358","type":"print"},{"value":"1440-1797","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2026-05-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70296"}}