{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T09:33:32Z","timestamp":1771493612683,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,1,7]],"date-time":"2014-01-07T00:00:00Z","timestamp":1389052800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuropathology"],"published-print":{"date-parts":[[2014,6]]},"abstract":"Temozolomide (TMZ<\/jats:styled-content>) is an oral alkylating agent which is widely used in the treatment of glioblastoma (GBM<\/jats:styled-content>) and is composed of astrocytic and\/or oligodendroglial tumors, and the evaluation of O6<\/jats:sup><\/jats:styled-content>\u2010methylguanine DNA<\/jats:styled-content> methyltransferase (MGMT<\/jats:styled-content>) expression is important to predict the response to TMZ<\/jats:styled-content> therapy. In this study, we conducted immunohistochemical analysis of 117 cases of Japanese GBM<\/jats:styled-content> including 19 cases of GBM<\/jats:styled-content> with oligodendroglioma component (GBMO<\/jats:styled-content>), using a scoring system for quantitative evaluation of staining intensity and proportion of MGMT<\/jats:styled-content>, and performed survival analysis of these patients. Immunohistochemically, 55 cases (47%) were positive for MGMT<\/jats:styled-content> with various intensities and proportions (total score (TS<\/jats:styled-content>)\u2009\u2265\u20092), while 62 cases (53%) were negative (TS<\/jats:styled-content>\u2009=\u20090). The distribution of MGMT<\/jats:styled-content> expression pattern was not affected by any clinicopathological parameters such as the histological subtype (GBM<\/jats:styled-content> vs. GBMO<\/jats:styled-content>), age and gender. The survival analysis of these patients revealed that the minimal expression of MGMT<\/jats:styled-content> (TS<\/jats:styled-content>\u2009\u2265\u20092) was a significant unfavorable prognostic factor (P<\/jats:italic>\u2009<\u20090.001) as well as resectability (P<\/jats:italic>\u2009=\u20090.004). Moreover, multivariate analysis showed that minimal MGMT<\/jats:styled-content> expression in GBM<\/jats:styled-content> was the most potent independent predictor for progression free survival (P<\/jats:italic>\u2009<\u20090.001) and also overall patient survival (P<\/jats:italic>\u2009<\u20090.001). This is the first report employing the scoring system for both staining intensity and proportion to evaluate immunohistochemical MGMT<\/jats:styled-content> expression in GBM<\/jats:styled-content>. In addition, our results emphases the clinicopathological values of the immunohistochemical approach for MGMT<\/jats:styled-content> expression in glioma patients as a routine laboratory examination.<\/jats:p>","DOI":"10.1111\/neup.12091","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T03:26:53Z","timestamp":1389151613000},"page":"268-276","source":"Crossref","is-referenced-by-count":20,"title":["Immunohistochemical evaluation of O6<\/sup><\/scp>\u2010methylguanine DNA<\/scp> methyltransferase (MGMT<\/scp>) expression in 117 cases of glioblastoma"],"prefix":"10.1111","volume":"34","author":[{"given":"Masaya","family":"Miyazaki","sequence":"first","affiliation":[{"name":"Department of Cancer Pathology Hokkaido University Graduate School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Nishihara","sequence":"additional","affiliation":[{"name":"Department of Translational Pathology Hokkaido 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