{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T21:04:18Z","timestamp":1792098258975,"version":"4.4.0"},"reference-count":16,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,9,1]],"date-time":"2014-09-01T00:00:00Z","timestamp":1409529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Ministry of Education, Culture, Sports, Science, and Technology of Japan and an Intramural Research Grant (22-7) for Neurological and Psychiatric Disorders of NCNP"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuropathology"],"published-print":{"date-parts":[[2015,2]]},"abstract":"The G<\/jats:styled-content>allyas method is a silver impregnation technique that is essential in the field of neuropathology because of its high sensitivity for the detection of argentophilic inclusion bodies in the central nervous system. In J<\/jats:styled-content>apan, the G<\/jats:styled-content>allyas method has improved and is widely used as the \u201cmodified G<\/jats:styled-content>allyas method\u201d. However, this method is not popularly used in general pathology laboratories because of the need for special reagents, several staining processes, and skilled techniques. The objective of the current study was to provide a simplified G<\/jats:styled-content>allyas method. We omitted the lanthanum nitrate step from the staining process and verified the adequacy in comparison with the original method as well as immunohistochemistry, using specimens from patients of A<\/jats:styled-content>lzheimer's disease, argyrophilic grain disease, multiple system atrophy, Pick's disease, and L<\/jats:styled-content>ewy body disease. The simplified method provided good staining to all the structures in archival tissues, compared with the modified G<\/jats:styled-content>allyas method in a significantly shorter staining time. The lanthanum nitrate step can be omitted from the modified G<\/jats:styled-content>allyas method, resulting in reduction in the number of reagents required and shortening of the staining time.<\/jats:p>","DOI":"10.1111\/neup.12144","type":"journal-article","created":{"date-parts":[[2014,9,2]],"date-time":"2014-09-02T03:01:15Z","timestamp":1409626875000},"page":"10-15","source":"Crossref","is-referenced-by-count":37,"title":["Simplification of the modified G<\/scp>allyas method"],"prefix":"10.1111","volume":"35","author":[{"given":"Nobuo","family":"Kuninaka","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine National Center Hospital of Neurology and Psychiatry Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minato","family":"Kawaguchi","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine National Center Hospital of Neurology and Psychiatry Tokyo 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