{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T06:05:15Z","timestamp":1777183515559,"version":"3.51.4"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,7,29]],"date-time":"2019-07-29T00:00:00Z","timestamp":1564358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neuropathology"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:p>We report a case of a young female patient who developed progressive neurological dysfunction with a ring\u2010enhancing tumor\u2010like nodule on brain magnetic resonance imaging. Urgent surgery was performed to remove the mass in the left basal ganglia. Pathological findings showed that the necrotic brain areas were accompanied by congestion, edema, discrete hemorrhage, and intestinal and perivascular lymphohistiocytic infiltration. Immunohistochemical staining results showed that <jats:italic>Toxoplasma gondii (T. gondii)<\/jats:italic> immunoreactivity was detected in both cysts and tachyzoites in these areas. The glycerol\u20103\u2010phosphate dehydrogenase gene <jats:italic>(B1)<\/jats:italic> of <jats:italic>T. gondii<\/jats:italic> was amplified by sequence\u2010specific polymerase chain reaction (PCR) and the PCR products were bi\u2010directional Sanger sequenced. A 195\u2009bp consensus sequence of the gene <jats:italic>B1<\/jats:italic> was found to be 98% identical to a reference <jats:italic>T. gondii<\/jats:italic> sequence (GenBank accession No. kx270373). The final diagnosis was toxoplasmic encephalitis in the left basal ganglia. This report suggests that PCR and bi\u2010directional DNA sequencing of <jats:italic>T. gondii<\/jats:italic> gene might be the most convenient and rapid tools for accurate diagnosis of toxoplasmic encephalitis .<\/jats:p>","DOI":"10.1111\/neup.12588","type":"journal-article","created":{"date-parts":[[2019,7,29]],"date-time":"2019-07-29T07:00:38Z","timestamp":1564383638000},"page":"398-403","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Toxoplasmic encephalitis of basal ganglia with tumor\u2010like features proven by pathogen\u2010specific polymerase chain reaction and direct DNA sequencing"],"prefix":"10.1111","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2167-0615","authenticated-orcid":false,"given":"Yunyun","family":"Li","sequence":"first","affiliation":[{"name":"Department of Pathology Changhai Hospital, the Second Military Medical University  Shanghai 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