{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T08:46:06Z","timestamp":1777020366305,"version":"3.51.4"},"reference-count":8,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T00:00:00Z","timestamp":1771891200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T00:00:00Z","timestamp":1771891200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neuropathology"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In order to prepare specimens to clarify the anatomical localization of intracerebral hematomas (ICHs), we examined 18 autopsy cases ranging from 1\u2009day to 2.5\u2009months after onset: Five cases of hypertensive ICH (two cases of putaminal hematoma, one case of thalamic hematoma, one case of mixed\u2010type hematoma, and one case of cerebellar hematoma), two cases of subarachnoid hemorrhage with ICH, three cases of cerebral amyloid angiopathy\u2010related hemorrhage, two cases of ICH associated with blood coagulation abnormalities, and six cases of brain death with ICH (brain death period: 10\u2009h to 13\u2009days). Paraffin blocks containing hematomas that have hardened and have poor formalin penetration can be easily sectioned at 6\u2009\u03bcm thickness by using Histoheme, an ammonia\u2010based softening reagent. By mounting the sections on MAS\u2010coated glass slides or New Silan III\u2010coated glass slides, we were able to minimize the peeling of hematomas from the slides. Conventional staining (Kl\u00fcver\u2013Barrera staining, Elastica\u2010Masson Goldner staining) showed decreased staining of the brain tissue surrounding the hematoma due to the effects of edema, making the localization of the hematoma unclear. Synaptophysin (SYP) immunostaining was visualized with 3,3\u2032\u2010diaminobenzidine tetrahydrochloride (DAB)\u2010CoCl\n                    <jats:sub>2<\/jats:sub>\n                    and double stained with eosin azur 50 (EA50) (DAB\u2010CoCl\n                    <jats:sub>2<\/jats:sub>\n                    SYP\u2009+\u2009EA50). This method revealed gray matter as black and fresh to relatively recent hematoma as red, and clearly demonstrated the anatomical localization of the hematoma without any reduction in staining due to edema. In contrast, hematomas in cases more than 29\u2009days after onset showed heterogeneous staining in red or green, and the boundary between the brain tissue and the hematoma was unclear. However, with double staining using DAB\u2010CoCl\n                    <jats:sub>2<\/jats:sub>\n                    SYP and Masson Goldner staining, the hematoma stained dark red, and the boundary with the brain tissue was more clearly visible than with DAB\u2010CoCl\n                    <jats:sub>2<\/jats:sub>\n                    SYP\u2009+\u2009EA50.\n                  <\/jats:p>","DOI":"10.1111\/neup.70051","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T18:14:24Z","timestamp":1772993664000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An Attempt of a Staining Method to Clarify the Localization of Hematomas in Autopsy Cases of Intracerebral Hemorrhage: Application of Double Staining With Immunohistochemistry for Anti\u2010Synaptophysin Antibody Using\n                    <scp>DAB<\/scp>\n                    \u2010\n                    <scp>\n                      CoCl\n                      <sub>2<\/sub>\n                    <\/scp>\n                    and\n                    <scp>EA50<\/scp>"],"prefix":"10.1111","volume":"46","author":[{"given":"Seiichi","family":"Hirata","sequence":"first","affiliation":[{"name":"Department of Pathology Niigata Neurosurgical Hospital  Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Niigata Neurosurgical Hospital  Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6845-1500","authenticated-orcid":false,"given":"Shigeki","family":"Takeda","sequence":"additional","affiliation":[{"name":"Department of Pathology Niigata Neurosurgical Hospital  Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1789.2003.00506.x"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1789.2011.01219.x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/neup.12476"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/neup.12998"},{"issue":"11","key":"e_1_2_11_6_1","first-page":"1949","article-title":"Research Report for 1985, Guidelines and Criteria for Determining Brain Death","volume":"94","author":"Ministry of Health and Welfare, Special Research Project on Science Research, Research Group on Brain Death","year":"1985","journal-title":"JMA Journal"},{"issue":"3","key":"e_1_2_11_7_1","first-page":"334","article-title":"Elastica\u2010Masson Stain: An Excellent Staining Method Designed for Morphological Examination of Vascular Lesions","volume":"28","author":"Watanabe M.","year":"2010","journal-title":"Byouri to Rinsho"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/30.10.6182185"},{"key":"e_1_2_11_9_1","first-page":"32","volume-title":"Non Neoplastic Disease Pathology Atlas. Central Nervous System","author":"Takeda S.","year":"2024"}],"container-title":["Neuropathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/neup.70051","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/neup.70051","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/neup.70051","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T08:04:14Z","timestamp":1777017854000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/neup.70051"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,24]]},"references-count":8,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1111\/neup.70051"],"URL":"https:\/\/doi.org\/10.1111\/neup.70051","archive":["Portico"],"relation":{},"ISSN":["0919-6544","1440-1789"],"issn-type":[{"value":"0919-6544","type":"print"},{"value":"1440-1789","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,24]]},"assertion":[{"value":"2025-11-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70051"}}