{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T08:40:22Z","timestamp":1792744822880,"version":"4.5.0"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T00:00:00Z","timestamp":1792281600000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neuropathology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The 2021 WHO Classification of Tumors of the Central Nervous System incorporates integrated molecular diagnostics into the classification of diffuse gliomas; however, access to comprehensive molecular testing remains limited in some settings. This study retrospectively evaluated grade\u2010associated patterns of PCR\u2010based loss of heterozygosity (LOH) and microsatellite instability (MSI) in a historical, morphology\u2010defined cohort of diffuse astrocytic gliomas from the pre\u2010IDH era. LOH and MSI analyses were performed using 14 LOH and 5 MSI microsatellite markers in 40 archival diffuse astrocytic gliomas, including 12 diffuse astrocytomas, NOS (WHO grade 2), 15 anaplastic astrocytomas, NOS (WHO grade 3), and 13 glioblastomas, NOS (WHO grade 4), collected between 1997 and 2002 prior to the availability of IDH immunohistochemistry. LOH\u2010high (LOH\u2010H) was defined as LOH involving three or more of the seven microsatellite markers examined at each locus, and MSI\u2010high (MSI\u2010H) as instability at two or more of the five markers. LOH was detected in 77.5% of tumors. LOH\u2010H at 10q23 (\n                    <jats:italic>PTEN<\/jats:italic>\n                    ) correlated significantly with WHO grade (8.3%, 40.0%, and 61.5% in grades 2, 3, and 4, respectively;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.022) and distinguished high\u2010grade from low\u2010grade tumors (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.015). LOH\u2010H at either 10q23 or 17p13 (\n                    <jats:italic>TP53<\/jats:italic>\n                    ) was observed in 67.9% of high\u2010grade tumors compared with 25.0% of grade 2 tumors (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.013). MSI and MSI\u2010H rates were 17.5% and 10.0%, respectively. Three of the four MSI\u2010H tumors lacked concurrent LOH\u2010H; given the small number of MSI\u2010H tumors, this observation is preliminary and should not be interpreted as evidence of distinct genomic instability pathways. LOH\u2010H at 10q23, alone or in combination with 17p13, was associated with higher morphology\u2010based WHO grade, whereas 17p13 LOH alone showed no significant grade association. Because IDH mutation status could not be determined, these findings should not be extrapolated to contemporary WHO\u2010defined astrocytic gliomas; whether these findings apply to molecularly defined gliomas would require validation.\n                  <\/jats:p>","DOI":"10.1111\/neup.70082","type":"journal-article","created":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:21:56Z","timestamp":1792369316000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Loss of Heterozygosity and Microsatellite Instability in Diffuse Astrocytic Gliomas: Retrospective Reinterpretation of\n                    <scp>PCR<\/scp>\n                    \u2010Based Molecular Data From a\n                    <scp>Pre\u2010IDH\u2010Era<\/scp>\n                    Korean Cohort"],"prefix":"10.1111","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2793-6856","authenticated-orcid":false,"given":"Na Rae","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Pathology Bucheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwan Ho","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The Veterans Health Service Medical Center  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi Jung","family":"Kwon","sequence":"additional","affiliation":[{"name":"Department of Pathology Sacred Heart Hospital, Hallym University College of Medicine  Anyang Gyeonggido South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4867-9003","authenticated-orcid":false,"given":"Gwangil","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pathology Bundang CHA Medical Center, CHA University  Seongnam Gyeonggido South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seong Jin","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Pathology Kangdong Sacred Heart Hospital, Hallym University College of Medicine  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,18]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/nou223"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/noaf177"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-016-1545-1"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/noab106"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/neup.12953"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/nop\/npae124"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2024.1378397"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature26000"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/neup.12970"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/bpa.13201"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-009-0595-z"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-004-0693-x"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0215(20000720)89:4<378::AID-IJC10>3.0.CO;2-T"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/noae107"},{"key":"e_1_2_11_16_1","first-page":"180","article-title":"Microsatellite Instability and Expression of DNA Mismatch Repair Genes in Malignant Astrocytic Tumors From Adult and Pediatric Patients","volume":"22","author":"Szybka M.","year":"2003","journal-title":"Clinical Neuropathology"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.2007.00049.x"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/bpa.12832"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa043330"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2015.30.11.1597"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bas.2023.101775"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers16162870"},{"key":"e_1_2_11_23_1","article-title":"Diagnostic Test Accuracy and Cost\u2010Effectiveness of Tests for Codeletion of Chromosomal Arms 1p and 19q in People With Glioma","volume":"3","author":"McAleenan A.","year":"2022","journal-title":"Cochrane Database 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