{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T00:38:08Z","timestamp":1771634288046,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,1,17]],"date-time":"2013-01-17T00:00:00Z","timestamp":1358380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background\u2002<\/jats:bold> Pharmacological studies using selective P2Y<jats:sub>1<\/jats:sub> antagonists, such as MRS2500, and studies with P2Y<jats:sub>1<\/jats:sub><jats:sup>\u2212\/\u2212<\/jats:sup> knockout mice have demonstrated that purinergic neuromuscular transmission is mediated by P2Y<jats:sub>1<\/jats:sub> receptors in the colon. The aim of the present study was to test whether P2Y<jats:sub>1<\/jats:sub> receptors are involved in purinergic neurotransmission in the antrum and cecum.<\/jats:p><jats:p><jats:bold>Methods\u2002<\/jats:bold> Microelectrode recordings were performed on strips from the antrum and cecum of wild type animals (WT) and P2Y<jats:sub>1<\/jats:sub><jats:sup>\u2212\/\u2212<\/jats:sup> mice.<\/jats:p><jats:p><jats:bold>Key Results\u2002<\/jats:bold> In the antrum, no differences in resting membrane potential and slow wave activity were observed between groups. In WT animals, electrical field stimulation elicited a MRS2500\u2010sensitive inhibitory junction potential (IJP). In P2Y<jats:sub>1<\/jats:sub><jats:sup>\u2212\/\u2212<\/jats:sup> mice, a nitrergic IJP (<jats:italic>N<\/jats:italic><jats:sup>\u03c9<\/jats:sup>\u2010nitro\u2010<jats:sc>l<\/jats:sc>\u2010arginine\u2010sensitive), but not a purinergic IJP was recorded. This IJP was equivalent to the response obtained in strips from WT animals previously incubated with MRS2500. Similar results were obtained in the cecum: 1\u2010 the purinergic IJP (MRS2500\u2010sensitive) recorded in WT animals was absent in P2Y<jats:sub>1<\/jats:sub><jats:sup>\u2212\/\u2212<\/jats:sup> mice 2\u2010 nitrergic neurotransmission was preserved in both groups. Moreover, 1\u2010 spontaneous IJP (MRS2500\u2010sensitive) could be recorded in WT, but not in P2Y<jats:sub>1<\/jats:sub><jats:sup>\u2212\/\u2212<\/jats:sup> mice 2\u2010 MRS2365 a P2Y<jats:sub>1<\/jats:sub> agonist caused smooth muscle hyperpolarization in WT, but not in P2Y<jats:sub>1<\/jats:sub><jats:sup>\u2212\/\u2212<\/jats:sup> animals, and 3\u2010 \u03b2\u2010NAD caused smooth muscle hyperpolarization both in WT and P2Y<jats:sub>1<\/jats:sub><jats:sup>\u2212\/\u2212<\/jats:sup> animals.<\/jats:p><jats:p><jats:bold>Conclusions &amp; Inferences\u2002<\/jats:bold> 1\u2010 P2Y<jats:sub>1<\/jats:sub> receptor is the general mechanism of purinergic inhibition in the gastrointestinal tract, 2\u2010 P2Y<jats:sub>1<\/jats:sub><jats:sup>\u2212\/\u2212<\/jats:sup> mouse is a useful animal model to study selective impairment of purinergic neurotransmission and 3\u2010 P2Y<jats:sub>1<\/jats:sub><jats:sup>\u2212\/\u2212<\/jats:sup> mouse might help in the identification of purinergic neurotransmitter(s).<\/jats:p>","DOI":"10.1111\/nmo.12060","type":"journal-article","created":{"date-parts":[[2013,1,17]],"date-time":"2013-01-17T10:18:33Z","timestamp":1358417913000},"source":"Crossref","is-referenced-by-count":36,"title":["P2Y<sub>1<\/sub> knockout mice lack purinergic neuromuscular transmission in the antrum and cecum"],"prefix":"10.1111","volume":"25","author":[{"given":"V.","family":"Gil","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Mart\u00ednez\u2010Cutillas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Ma\u00f1\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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