{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:01:30Z","timestamp":1791252090408,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,11,29]],"date-time":"2013-11-29T00:00:00Z","timestamp":1385683200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2014,3]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n Increased intestinal permeability and altered expression of tight junction (\n TJ<\/jats:styled-content>\n ) proteins may be implicated in the pathogenesis of irritable bowel syndrome (\n IBS<\/jats:styled-content>\n ). This study aimed to investigate the expression of adherens junction (\n AJ<\/jats:styled-content>\n ) protein E\u2010cadherin and\n TJ<\/jats:styled-content>\n proteins zonula occludens (\n ZO<\/jats:styled-content>\n )\u20101 and claudin (\n CLD<\/jats:styled-content>\n )\u20101 and associations with\n IBS<\/jats:styled-content>\n symptoms.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Junctional proteins were immunostained in cecal biopsy tissue of Rome II\n IBS<\/jats:styled-content>\n patients (\n n<\/jats:italic>\n \u00a0=\u00a034) comprising both alternating (\n IBS<\/jats:styled-content>\n \u2010A) and diarrhea predominant (\n IBS<\/jats:styled-content>\n \u2010D) subtypes, and controls (\n n<\/jats:italic>\n \u00a0=\u00a012).\n IBS<\/jats:styled-content>\n symptom duration, abdominal pain severity and stool frequency were assessed for\n IBS<\/jats:styled-content>\n patients. Protein expression was determined by immunofluorescence.\n <\/jats:p>\n <\/jats:sec>\n \n Key Results<\/jats:title>\n \n E\u2010cadherin and\n ZO<\/jats:styled-content>\n \u20101 protein expression was significantly lower (\n p<\/jats:italic>\n \u00a0=\u00a00.03 and\n p<\/jats:italic>\n \u00a0=\u00a00.016, respectively) in the cecal surface epithelium of the\n IBS<\/jats:styled-content>\n group comprising both\n IBS<\/jats:styled-content>\n \u2010A and\n IBS<\/jats:styled-content>\n \u2010D subtypes.\n CLD<\/jats:styled-content>\n \u20101 expression was not significantly altered compared with controls. On subtype analysis,\n ZO<\/jats:styled-content>\n \u20101 expression was significantly reduced in both\n IBS<\/jats:styled-content>\n \u2010A and\n IBS<\/jats:styled-content>\n \u2010D compared with controls, whereas E\u2010cadherin was reduced only in\n IBS<\/jats:styled-content>\n \u2010A. Lower E\u2010cadherin expression was associated with longer symptoms duration specifically in\n IBS<\/jats:styled-content>\n \u2010A patients (\n r<\/jats:italic>\n s<\/jats:sub>\n \u00a0=\u00a0\u22120.76,\n p<\/jats:italic>\n \u00a0=\u00a00.004). Reduced E\u2010cadherin associated with abdominal pain severity in the overall\n IBS<\/jats:styled-content>\n group (\n r<\/jats:italic>\n s<\/jats:sub>\n \u00a0=\u00a0\u22120.36,\n p<\/jats:italic>\n \u00a0=\u00a00.041), but this association was unrelated to\n IBS<\/jats:styled-content>\n subtype.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions & Inferences<\/jats:title>\n \n E\u2010cadherin protein expression in the cecum was significantly lower in\n IBS<\/jats:styled-content>\n \u2010A compared with controls and associated with longstanding symptoms. E\u2010cadherin was further associated with abdominal pain severity in the\n IBS<\/jats:styled-content>\n group overall, but unrelated to\n IBS<\/jats:styled-content>\n subtype. Altered E\u2010cadherin expression may provide novel insights into mechanisms underlying intestinal barrier dysfunction in\n IBS<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/nmo.12262","type":"journal-article","created":{"date-parts":[[2013,11,29]],"date-time":"2013-11-29T05:52:41Z","timestamp":1385704361000},"page":"316-325","source":"Crossref","is-referenced-by-count":52,"title":["Reduced E\u2010cadherin expression is associated with abdominal pain and symptom duration in a study of alternating and diarrhea predominant\n IBS<\/scp>"],"prefix":"10.1111","volume":"26","author":[{"given":"E.","family":"Wilcz\u2010Villega","sequence":"first","affiliation":[{"name":"Department of Clinical Medicine Trinity Centre for Health Sciences Adelaide & Meath Hospital Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"McClean","sequence":"additional","affiliation":[{"name":"Centre for Microbial Host Interactions Institute of 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