{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T06:15:48Z","timestamp":1696572948257},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,4,23]],"date-time":"2014-04-23T00:00:00Z","timestamp":1398211200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Although without evidence of organic structural abnormalities, pain or discomfort is a prominent symptom of functional dyspepsia and considered to reflect visceral hypersensitivity whose underlying mechanism is poorly understood. Here, we studied electrophysiological properties and expression of voltage\u2010gated potassium channels in dorsal root ganglion (<jats:styled-content style=\"fixed-case\">DRG<\/jats:styled-content>) neurons in a rat model of functional dyspepsia induced by neonatal gastric irritation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Male <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>prague\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>awley rat pups at 10\u2010day old received 0.1% iodoacetamide (<jats:styled-content style=\"fixed-case\">IA<\/jats:styled-content>) or vehicle by oral gavage for 6\u00a0days and studied at adulthood. Retrograde tracer\u2010labeled gastric\u2010specific T<jats:sub>8<\/jats:sub>\u2013T<jats:sub>12<\/jats:sub> <jats:styled-content style=\"fixed-case\">DRG<\/jats:styled-content> neurons were harvested for the patch\u2010clamp study in voltage and current\u2010clamp modes and protein expression of K<jats:sup>+<\/jats:sup> channel in T<jats:sub>8<\/jats:sub>\u2013T<jats:sub>12<\/jats:sub> <jats:styled-content style=\"fixed-case\">DRG<\/jats:styled-content>s was examined by western blotting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>(1) Gastric specific but not non\u2010gastric <jats:styled-content style=\"fixed-case\">DRG<\/jats:styled-content> neurons showed an enhanced excitability in neonatal <jats:styled-content style=\"fixed-case\">IA<\/jats:styled-content>\u2010treated rats compared to the control: depolarized resting membrane potentials, a lower current threshold for action potential (<jats:styled-content style=\"fixed-case\">AP<\/jats:styled-content>) activation, and an increase in the number of <jats:styled-content style=\"fixed-case\">AP<\/jats:styled-content>s in response to current stimulation. (2) The current density of tetraethylammonium insensitive (transiently inactivating A\u2010type current), but not the tetraethylammonium sensitive (slow\u2010inactivating delayed rectifier K<jats:sup>+<\/jats:sup> currents), was significantly smaller in <jats:styled-content style=\"fixed-case\">IA<\/jats:styled-content>\u2010treated rats (65.4\u00a0\u00b1\u00a06.9\u00a0pA\/pF), compared to that of control (93.1\u00a0\u00b1\u00a08.3\u00a0pA\/pF). (3) Protein expression of K<jats:sub>V<\/jats:sub>4.3 was down\u2010regulated in <jats:styled-content style=\"fixed-case\">IA<\/jats:styled-content>\u2010treated rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>A\u2010type potassium channels are significantly down\u2010regulated in the gastric\u2010specific <jats:styled-content style=\"fixed-case\">DRG<\/jats:styled-content> neurons in adult rats with mild neonatal gastric irritation, which in part contribute to the enhanced <jats:styled-content style=\"fixed-case\">DRG<\/jats:styled-content> neuron excitabilities that leads to the development of gastric hypersensitivity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12350","type":"journal-article","created":{"date-parts":[[2014,4,24]],"date-time":"2014-04-24T03:18:36Z","timestamp":1398309516000},"page":"962-970","source":"Crossref","is-referenced-by-count":4,"title":["Down\u2010regulation of A\u2010type potassium channel in gastric\u2010specific <scp>DRG<\/scp> neurons in a rat model of functional dyspepsia"],"prefix":"10.1111","volume":"26","author":[{"given":"S.","family":"Li","sequence":"first","affiliation":[{"name":"Veterans Research and Education Foundation VA Medical Center Oklahoma City OK USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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