{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T19:49:59Z","timestamp":1788637799948,"version":"build-2803163510"},"reference-count":49,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,5,25]],"date-time":"2014-05-25T00:00:00Z","timestamp":1400976000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"FWO","award":["G.0A44.13; G.0889.11"],"award-info":[{"award-number":["G.0A44.13; G.0889.11"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Correct neuronal identification is essential to study neurons in health and disease. Although commonly used as pan\u2010neuronal marker, HuC\/D's expression pattern varies substantially between healthy and (patho)physiological conditions. This heterogenic labeling has received very little attention. We sought to investigate the subcellular HuC\/D localization in enteric neurons in different conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The localization of neuronal <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010binding proteins HuC\/D was investigated by immunohistochemistry in the mouse myenteric plexus using different toxins and caustic agents. Preparations were also stained with Sox10 and glial fibrillary acidic protein (<jats:styled-content style=\"fixed-case\">GFAP<\/jats:styled-content>) antibodies to assess enteric glial cell appearance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Mechanically induced tissue damage, interference with the respiratory chain and oxygen (<jats:styled-content style=\"fixed-case\"><jats:roman>O<\/jats:roman><\/jats:styled-content><jats:sub>2<\/jats:sub>) deprivation increased nuclear HuC\/D immunoreactivity. This effect was paralleled by a distortion of the <jats:styled-content style=\"fixed-case\">GFAP<\/jats:styled-content>\u2010labeled glial network along with a loss of Sox10 expression and coincided with the activation of a non\u2010apoptotic genetic program. Chemically induced damage and specific noxious stimuli did not induce a change in HuC\/D immunoreactivity, supporting the specific nature of the nuclear HuC\/D localization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>HuC\/D is not merely a pan\u2010neuronal marker but its subcellular localization also reflects the condition of a neuron at the time of fixation. The functional meaning of this change in HuC\/D localization is not entirely clear, but disturbance in <jats:styled-content style=\"fixed-case\"><jats:roman>O<\/jats:roman><\/jats:styled-content><jats:sub>2<\/jats:sub> supply in combination with the support of enteric glial cells seems to play a crucial role. The molecular consequence of changes in HuC\/D expression needs to be further investigated.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12371","type":"journal-article","created":{"date-parts":[[2014,5,26]],"date-time":"2014-05-26T02:55:07Z","timestamp":1401072907000},"page":"1131-1143","source":"Crossref","is-referenced-by-count":52,"title":["Distinct subcellular localization of the neuronal marker HuC\/D reveals hypoxia\u2010induced damage in enteric neurons"],"prefix":"10.1111","volume":"26","author":[{"given":"A.\u2010S.","family":"Desmet","sequence":"first","affiliation":[{"name":"Laboratory for Enteric NeuroScience (LENS) Translational Research Center for GastroIntestinal Disorders (TARGID) University of Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Cirillo","sequence":"additional","affiliation":[{"name":"Laboratory for Enteric NeuroScience (LENS) Translational Research Center for GastroIntestinal Disorders (TARGID) University of Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Vanden Berghe","sequence":"additional","affiliation":[{"name":"Laboratory for Enteric NeuroScience (LENS) Translational Research Center for GastroIntestinal Disorders (TARGID) University of Leuven Leuven 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