{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:31:29Z","timestamp":1788870689801,"version":"build-2803163510"},"reference-count":25,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,9,8]],"date-time":"2014-09-08T00:00:00Z","timestamp":1410134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001279","name":"Great Ormond Street Hospital Charity","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001279","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2014,10]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n Reliable methods of labeling human enteric nervous system (\n ENS<\/jats:styled-content>\n ) stem cells for use in novel cell replacement therapies for enteric neuropathies are lacking. Here, we explore the possibility of using lentiviral vectors expressing fluorescent reporter genes to transduce, label, and trace mouse and human\n ENS<\/jats:styled-content>\n stem cells following transplantation into mouse gut.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Enteric nervous system precursors, including ENS stem cells, were isolated from enzymatically dissociated mouse and human gut tissues. Lentivirus containing e\n GFP<\/jats:styled-content>\n or mCherry fluorescent reporter genes was added to gut cell cultures at a multiplicity of infection of 2\u20135. After fluorescence activated cell sorting for e\n GFP<\/jats:styled-content>\n and subsequent analysis with markers of proliferation and cell phenotype, transduced mouse and human cells were transplanted into the gut of C57\n BL<\/jats:styled-content>\n \/6 and immune deficient Rag2\u2010\/gamma chain\u2010\/C5 mice, respectively and analyzed up to 60\u00a0days later.\n <\/jats:p>\n <\/jats:sec>\n \n Key Results<\/jats:title>\n \n Mouse and human transduced cells survived\n in vitro<\/jats:italic>\n , maintained intense e\n GFP<\/jats:styled-content>\n expression, proliferated as shown by BrdU incorporation, and formed characteristic neurospheres. When transplanted into mouse gut\n in vivo<\/jats:italic>\n and analyzed up to 2\u00a0months later, transduced mouse and human cells survived, strongly expressed e\n GFP<\/jats:styled-content>\n and integrated into endogenous\n ENS<\/jats:styled-content>\n networks.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions & Inferences<\/jats:title>\n \n Lentiviral vectors expressing fluorescent reporter genes enable efficient, stable, long\u2010term labeling of\n ENS<\/jats:styled-content>\n stem cells when transplanted into\n in vivo<\/jats:italic>\n mouse gut. This lentiviral approach not only addresses the need for a reliable fluorescent marker of human\n ENS<\/jats:styled-content>\n stem cells for preclinical studies, but also raises the possibility of using lentiviruses for other applications, such as gene therapy.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/nmo.12420","type":"journal-article","created":{"date-parts":[[2014,9,8]],"date-time":"2014-09-08T22:41:37Z","timestamp":1410216097000},"page":"1513-1518","source":"Crossref","is-referenced-by-count":22,"title":["Lentiviral labeling of mouse and human enteric nervous system stem cells for regenerative medicine studies"],"prefix":"10.1111","volume":"26","author":[{"given":"D.","family":"Natarajan","sequence":"first","affiliation":[{"name":"Stem Cells and Regenerative Medicine UCL Institute of Child Health London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Cooper","sequence":"additional","affiliation":[{"name":"Stem Cells and Regenerative Medicine UCL Institute of Child Health London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Choudhury","sequence":"additional","affiliation":[{"name":"Stem Cells and Regenerative Medicine UCL Institute of Child Health London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.\u2010M.","family":"Delalande","sequence":"additional","affiliation":[{"name":"Stem Cells and Regenerative Medicine UCL Institute of Child Health London UK"},{"name":"Centre for Digestive Diseases Barts & The London School of Medicine & Dentistry Queen Mary, University of London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"McCann","sequence":"additional","affiliation":[{"name":"Stem Cells and Regenerative Medicine UCL Institute of Child Health London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. J.","family":"Howe","sequence":"additional","affiliation":[{"name":"Molecular and Cellular Immunology UCL Institute of Child Health London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Thapar","sequence":"additional","affiliation":[{"name":"Stem Cells and Regenerative Medicine UCL Institute of Child Health London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. J.","family":"Burns","sequence":"additional","affiliation":[{"name":"Stem Cells and Regenerative Medicine UCL Institute of Child Health London UK"},{"name":"Department of Clinical Genetics Erasmus MC Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,9,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2012.234"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/wdev.57"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/cge.12054"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2013.226"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2011.09.004"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.sempedsurg.2009.07.008"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2012.01.012"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00857"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2006.094565"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2009.02.048"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI65963"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2137"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0b013e3181a15d62"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-1-4"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1089\/10430340252898984"},{"key":"e_1_2_10_17_1","first-page":"85","article-title":"Vector systems for prenatal gene therapy: principles of retrovirus vector design and production","volume":"891","author":"Howe SJ","year":"2012","journal-title":"Methods Mol Biol"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-386509-0.00003-X"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.10060"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-013-8430-x"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12033-007-0010-8"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2009.319"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20120146"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80583-8"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2013.05.019"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.2174\/156652311794940782"}],"container-title":["Neurogastroenterology & Motility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnmo.12420","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nmo.12420","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T23:57:35Z","timestamp":1696204655000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/nmo.12420"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,8]]},"references-count":25,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2014,10]]}},"alternative-id":["10.1111\/nmo.12420"],"URL":"https:\/\/doi.org\/10.1111\/nmo.12420","archive":["Portico"],"relation":{},"ISSN":["1350-1925","1365-2982"],"issn-type":[{"value":"1350-1925","type":"print"},{"value":"1365-2982","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,8]]}}}