{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:16:00Z","timestamp":1787015760438,"version":"build-2736575974"},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,11,27]],"date-time":"2014-11-27T00:00:00Z","timestamp":1417046400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2015,1]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n Serotonin type 3 receptor (5\u2010\n HT<\/jats:styled-content>\n 3<\/jats:sub>\n R) antagonists are potentially useful therapeutic agents for diarrhea\u2010predominant irritable bowel syndrome (\n IBS<\/jats:styled-content>\n \u2010D). To identify biomarkers predicting effectiveness of the 5\u2010\n HT<\/jats:styled-content>\n 3<\/jats:sub>\n R antagonist (ramosetron) in\n IBS<\/jats:styled-content>\n \u2010D.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Irritable bowel syndrome\u2010D Japanese subjects received 2.5 or 5\u00a0\n \u03bc<\/jats:italic>\n g of ramosetron once daily for 4\u00a0weeks. Colonic mucosal\n S100A<\/jats:italic>\n and tryptophan hydroxylase (\n \n TPH<\/jats:styled-content>\n <\/jats:italic>\n )\n mRNA<\/jats:styled-content>\n expression levels were measured before treatment. Genomic\n DNA<\/jats:styled-content>\n was extracted from blood and polymorphisms of\n \n TPH<\/jats:styled-content>\n 1\n <\/jats:italic>\n and\n \n TPH<\/jats:styled-content>\n 2\n <\/jats:italic>\n were analyzed.\n <\/jats:p>\n <\/jats:sec>\n \n Key Results<\/jats:title>\n \n Forty\u2010two patients (27 men and 15 women, mean age 42\u00a0years) with\n IBS<\/jats:styled-content>\n \u2010D were included for analysis. Improvement of\n IBS<\/jats:styled-content>\n symptoms was seen in 26 (61.9%). Baseline\n S100A10<\/jats:italic>\n (\n p<\/jats:italic>\n \u00a0=\u00a00.02) and\n \n TPH<\/jats:styled-content>\n 1\n <\/jats:italic>\n (\n p<\/jats:italic>\n \u00a0=\u00a00.02) expression were significantly higher in the ramosetron responders than in the non\u2010responders. The frequencies of the\n \n TPH<\/jats:styled-content>\n 1\n <\/jats:italic>\n rs4537731G allele in linkage disequilibrium with the\n \n TPH<\/jats:styled-content>\n 1\n <\/jats:italic>\n rs7130929 T allele (11.5%\n vs<\/jats:italic>\n 50%,\n p<\/jats:italic>\n \u00a0=\u00a00.003;\n OR<\/jats:styled-content>\n : 12; 95% CI: 2.1\u201369) along with\n \n TPH<\/jats:styled-content>\n 1\n <\/jats:italic>\n rs211105 C allele (3.8%\n vs<\/jats:italic>\n 43.8%,\n p<\/jats:italic>\n \u00a0=\u00a00.0003;\n OR<\/jats:styled-content>\n : 19; 95% CI: 2.1\u2013181) were significantly lower in the responders than in the non\u2010responders. The mean scores of diarrhea at baseline were significantly higher (5.2\n vs<\/jats:italic>\n 3.7,\n p<\/jats:italic>\n \u00a0=\u00a00.005) in patients with\n \n TPH<\/jats:styled-content>\n 1\n <\/jats:italic>\n rs211105 T\/T than those with the G allele.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions & Inferences<\/jats:title>\n \n \n TPH<\/jats:styled-content>\n 1\n <\/jats:italic>\n gene polymorphisms and\n S100A10<\/jats:italic>\n expression, which correlate with 5\u2010\n HT<\/jats:styled-content>\n signaling were associated with ramosetron effectiveness in\n IBS<\/jats:styled-content>\n \u2010D, and may possibly lead to prospective identification of the resistance to treatment.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/nmo.12473","type":"journal-article","created":{"date-parts":[[2014,11,27]],"date-time":"2014-11-27T01:36:45Z","timestamp":1417052205000},"page":"82-91","source":"Crossref","is-referenced-by-count":20,"title":["Pilot study of Biomarkers for predicting effectiveness of ramosetron in diarrhea\u2010predominant irritable bowel syndrome: expression of\n S100A10<\/i>\n and polymorphisms of\n \n TPH<\/scp>\n 1\n <\/i>"],"prefix":"10.1111","volume":"27","author":[{"given":"A.","family":"Shiotani","sequence":"first","affiliation":[{"name":"Department of Internal Medicine Kawasaki Medical School Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Kusunoki","sequence":"additional","affiliation":[{"name":"Department of Comprehensive Medicine Kawasaki Medical School Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Ishii","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Kawasaki Medical School Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Imamura","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology and Laboratory Medicine Kawasaki Medical School Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Manabe","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology and Laboratory Medicine Kawasaki Medical School Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Kamada","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Kawasaki Medical School Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Hata","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology and Laboratory Medicine Kawasaki Medical School Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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