{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:24:14Z","timestamp":1791350654196,"version":"4.3.1"},"reference-count":46,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,5,9]],"date-time":"2015-05-09T00:00:00Z","timestamp":1431129600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology in Japan","award":["24590950"],"award-info":[{"award-number":["24590950"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Enteric glial cells (<jats:styled-content style=\"fixed-case\">EGC<\/jats:styled-content>s) play important roles in enteric integrity and regulation of gastrointestinal function. However, whether <jats:styled-content style=\"fixed-case\">EGC<\/jats:styled-content>s undergo pathophysiological changes in stress\u2010associated gastrointestinal disorders is unknown. We investigated structural and functional alterations in colonic <jats:styled-content style=\"fixed-case\">EGC<\/jats:styled-content>s and their roles in colonic contraction in an irritable bowel syndrome (<jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content>) model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>As a chronic stress, male Wistar rats underwent 3\u2010h maternal separation during postnatal days 2\u201314. As an acute stress, we used water\u2010immersion stress (4\u00a0h) in adulthood (at 8\u00a0weeks). We quantitatively and morphologically evaluated enteric neurons and <jats:styled-content style=\"fixed-case\">EGC<\/jats:styled-content>s using whole\u2010mount longitudinal muscle\u2010myenteric plexus preparations. Colonic contraction was analyzed with electrical field stimulation (<jats:styled-content style=\"fixed-case\">EFS<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Glial fibrillary acidic protein (<jats:styled-content style=\"fixed-case\">GFAP<\/jats:styled-content>) expression and the number of total, cholinergic, and nitrergic neurons were unchanged in maternally separated rats with acute stress (combined stress: an <jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content> model) compared with controls. However, the density of <jats:styled-content style=\"fixed-case\">GFAP<\/jats:styled-content>\u2010positive <jats:styled-content style=\"fixed-case\">EGC<\/jats:styled-content> processes that apparently overlapped with the neurons and the extent of bulbous swelling of terminals increased according to the stress intensity: control, acute stress, maternal separation, and combined stress. <jats:styled-content style=\"fixed-case\">EFS<\/jats:styled-content>\u2010induced colonic contractions were significantly greater in the combined stress rats than in controls. Higher dose of fluorocitrate, a selective inhibitor of <jats:styled-content style=\"fixed-case\">EGC<\/jats:styled-content> metabolism, was required to inhibit both <jats:styled-content style=\"fixed-case\">EFS<\/jats:styled-content>\u2010induced contraction and <jats:styled-content style=\"fixed-case\">EGC<\/jats:styled-content>s activation in the combined stress rats than in controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>Colonic <jats:styled-content style=\"fixed-case\">EGC<\/jats:styled-content>s exhibited structural alterations according to the stress intensity. <jats:styled-content style=\"fixed-case\">EGC<\/jats:styled-content>s were associated with stress\u2010induced colonic hyper\u2010contraction in the combined stress rats, which may underlie the pathogenesis of <jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12577","type":"journal-article","created":{"date-parts":[[2015,5,9]],"date-time":"2015-05-09T06:03:14Z","timestamp":1431151394000},"page":"1010-1023","source":"Crossref","is-referenced-by-count":43,"title":["Enteric glial cells are associated with stress\u2010induced colonic hyper\u2010contraction in maternally separated rats"],"prefix":"10.1111","volume":"27","author":[{"given":"Y.","family":"Fujikawa","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Osaka City University Graduate School of Medicine Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Tominaga","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Osaka City University Graduate School of Medicine Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Osaka City University Graduate School of Medicine Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Tanigawa","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Osaka City University Graduate School of Medicine Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Osaka City University Graduate School of Medicine Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Fujiwara","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Osaka City University Graduate School of Medicine Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Arakawa","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Osaka City University Graduate School of Medicine Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,5,9]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2012.32"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2006.03.008"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(00)70170-3"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2003.50103"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.11.022"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2009.02.074"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2009.01427.x"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-011-0379-9"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2007.01722.x"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.01.032"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2012.138"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2010.188409"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2007.01.051"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2009.01302.x"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2008.12.058"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81571-8"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2005.067595"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00067.2006"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2010.11.037"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-013-1102-0"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2008.06.008"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00182.2011"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2010.03.003"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2013.03.032"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(85)80074-6"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2000.278.4.G644"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.3109\/10253890.2011.558604"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00314.2002"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2009.01339.x"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1136(199709)21:1<106::AID-GLIA12>3.0.CO;2-W"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2003.08.051"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2008.01.020"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.2174\/1876386300902010011"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12029"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22231"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-3150.2004.00474.x"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004410050891"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2003.012625"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007677003387"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429-012-0500-8"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00221-011-2881-y"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2009.01467.x"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2012.04.021"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1097\/WNR.0b013e3280bef7f8"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2008.01238.x"},{"key":"e_1_2_11_47_1","first-page":"G693","article-title":"Evidence for a glutamatergic neural pathway in the myenteric plexus","volume":"261","author":"Wiley JW","year":"1991","journal-title":"Am J Physiol"}],"container-title":["Neurogastroenterology &amp; Motility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnmo.12577","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nmo.12577","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T12:50:34Z","timestamp":1696251034000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/nmo.12577"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,9]]},"references-count":46,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2015,7]]}},"alternative-id":["10.1111\/nmo.12577"],"URL":"https:\/\/doi.org\/10.1111\/nmo.12577","archive":["Portico"],"relation":{},"ISSN":["1350-1925","1365-2982"],"issn-type":[{"value":"1350-1925","type":"print"},{"value":"1365-2982","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,9]]}}}