{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T16:20:46Z","timestamp":1789143646744,"version":"build-2803163510"},"reference-count":54,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,5,13]],"date-time":"2015-05-13T00:00:00Z","timestamp":1431475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Local release of mast cell proteases during gastrointestinal surgery is associated with the inhibition of motility and postoperative ileus (<jats:styled-content style=\"fixed-case\">POI<\/jats:styled-content>). We determined whether activation of intramuscular mast cell affects the motor patterns of the human ileum and colon and whether proteases are involved.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Motor response of ileal and colonic circular muscle strips was measured in organ bath. Mast cell degranulation was induced by compound 48\/80 (c48\/80; 25\u2013675\u00a0<jats:italic>\u03bc<\/jats:italic>g\/mL). Motor response was quantified as tone, rhythmic phasic contractions (<jats:styled-content style=\"fixed-case\">RPC<\/jats:styled-content>s) and contractions to electric field stimulation (<jats:styled-content style=\"fixed-case\">EFS<\/jats:styled-content>; 40\u00a0Hz), and\u00a0bethanechol\u2010evoked contractions. Ketotifen (10<jats:sup>\u22126<\/jats:sup>\u00a0mol\/L) and a protease inhibitor cocktail (P8340) were used to evaluate the role of mast cell mediators.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>(a) c48\/80 impaired the spontaneous and the electrically evoked motor response in small bowel and colonic strips (sigmoid colon <jats:styled-content style=\"fixed-case\">EC<\/jats:styled-content><jats:sub>50<\/jats:sub>: 460.0\u00a0<jats:italic>\u03bc<\/jats:italic>g\/mL for <jats:styled-content style=\"fixed-case\">RPC<\/jats:styled-content>s and 8.9\u00a0<jats:italic>\u03bc<\/jats:italic>g\/mL for electrically evoked contraction amplitudes) and bethanechol\u2010evoked contractions. (b) Preincubation with ketotifen (10<jats:sup>\u22126<\/jats:sup>\u00a0mol\/L, 1\u00a0h) prevented the impairment of <jats:styled-content style=\"fixed-case\">RPC<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">EFS<\/jats:styled-content>\u2010evoked contractions in the sigmoid colon and ileum but not in the right colon. (c) Preincubation with P8340 also prevented the impairment of contractions in the sigmoid colon but not in the ileum or the right colon.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>Mast cell degranulation by c48\/80 inhibits the spontaneous and the nerve\u2010mediated motor response in the human ileum and colon. The effect is partially mediated by mast cell proteases and could be relevant in the pathophysiology of <jats:styled-content style=\"fixed-case\">POI<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12589","type":"journal-article","created":{"date-parts":[[2015,5,13]],"date-time":"2015-05-13T12:18:59Z","timestamp":1431519539000},"page":"1098-1109","source":"Crossref","is-referenced-by-count":8,"title":["Mast cell degranulation inhibits motor patterns of human ileum and sigmoid colon <i>in\u00a0vitro<\/i>: relevance for postoperative ileus"],"prefix":"10.1111","volume":"27","author":[{"given":"J.","family":"Rychter","sequence":"first","affiliation":[{"name":"CIBERehd, Centro de Investigaci\u00f3n Biom\u00e9dica en Red de enfermedades hep\u00e1ticas y digestivas Instituto de Salud Carlos III Barcelona Catalonia Spain"},{"name":"Department of Cell Biology, Physiology and Immunology Veterinary School Universitat Aut\u00f2noma de Barcelona Barcelona Catalonia 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