{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T22:15:14Z","timestamp":1791411314195,"version":"4.3.3"},"reference-count":19,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,5,31]],"date-time":"2015-05-31T00:00:00Z","timestamp":1433030400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Funded The Jessica Carr Foundation and the CHEO Research Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2015,8]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>Rett syndrome (RTT<\/jats:styled-content>) is an intellectual deficit and movement disorder that develops during early childhood in girls. Affected children are normal until 6\u201318\u00a0months of age, after which symptoms begin to appear. Most cases of RTT<\/jats:styled-content> are due to mutations in the MeCP<\/jats:styled-content>2 gene leading to disruption of neuronal communication in the central nervous system. In addition, RTT<\/jats:styled-content> patients show peripheral ailments such as gastrointestinal (GI<\/jats:styled-content>), respiratory, and cardiac dysfunction. The etiology of intestinal dysfunction in RTT<\/jats:styled-content> is not well\u2010understood. Reports on the presence of MeCP<\/jats:styled-content>2 in the peripheral nervous system are scant. As such we examined the levels of MeCP<\/jats:styled-content>2 in human and murine GI<\/jats:styled-content> tissue and assessed MeCP<\/jats:styled-content>2 expression at various developmental stages.<\/jats:p><\/jats:sec>Methods<\/jats:title>Immunohistochemistry for MeCP<\/jats:styled-content>2, HuC\/D, juvenile beta tubulin, and GFAP<\/jats:styled-content> was performed on human and murine intestine. Western blots of these same tissues were probed with MeCP<\/jats:styled-content>2, vAChT<\/jats:styled-content>, nNOS<\/jats:styled-content>, and beta\u2010actin antibodies.<\/jats:p><\/jats:sec>Key Results<\/jats:title>MeCP<\/jats:styled-content>2 is expressed throughout the GI<\/jats:styled-content> tract. MeCP<\/jats:styled-content>2 is expressed specifically in the enteric nervous system of the GI<\/jats:styled-content> tract. MeCP<\/jats:styled-content>2 is expressed in the GI<\/jats:styled-content> tract throughout development with appearance beginning at or before E11.5 in the murine intestine.<\/jats:p><\/jats:sec>Conclusions & Inferences<\/jats:title>The proof of MeCP<\/jats:styled-content>2 expression in enteric neurons suggests that the GI<\/jats:styled-content> dysmotility in Rett may arise from enteric network dysfunction secondary to MeCP<\/jats:styled-content>2 mutation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12605","type":"journal-article","created":{"date-parts":[[2015,6,1]],"date-time":"2015-06-01T00:49:44Z","timestamp":1433119784000},"page":"1156-1161","source":"Crossref","is-referenced-by-count":20,"title":["MeCP<\/scp>2 in the enteric nervous system"],"prefix":"10.1111","volume":"27","author":[{"given":"G.","family":"Wahba","sequence":"first","affiliation":[{"name":"Department of Cellular and Molecular Medicine University of Ottawa Ottawa ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. C.","family":"Schock","sequence":"additional","affiliation":[{"name":"Research Institute Children's Hospital of Eastern Ontario Ottawa ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Claridge","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Medicine University of Ottawa Ottawa ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Bettolli","sequence":"additional","affiliation":[{"name":"Departments of Surgery, Pathology and Paediatrics (Neurology) Children's Hospital of Eastern Ontario Ottawa ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Grynspan","sequence":"additional","affiliation":[{"name":"Departments of Surgery, Pathology and Paediatrics (Neurology) Children's Hospital of Eastern Ontario Ottawa ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Humphreys","sequence":"additional","affiliation":[{"name":"Departments of Surgery, Pathology and Paediatrics (Neurology) Children's Hospital of Eastern Ontario Ottawa ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. A.","family":"Staines","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Medicine University of Ottawa Ottawa ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,5,31]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0733-8619(02)00022-1"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.1320250506"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/13810"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-092910-154121"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/11.2.115"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2004.07.006"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2007.04.005"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1316-12.2012"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22009"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2005.05.056"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0887-8994(96)00218-4"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0b013e31824b6159"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0004-28032008000400005"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005176-199907000-00010"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1180"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.16.1.414"},{"key":"e_1_2_10_18_1","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1242\/dev.116.1.167","article-title":"A transgenic model for studying development of the enteric nervous system in normal and aganglionic mice","volume":"116","author":"Kapur RP","year":"1992","journal-title":"Development"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3053-11.2011"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9861(20000925)425:3<369::AID-CNE3>3.0.CO;2-7"}],"container-title":["Neurogastroenterology & Motility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnmo.12605","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nmo.12605","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T23:21:04Z","timestamp":1695511264000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/nmo.12605"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,31]]},"references-count":19,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2015,8]]}},"alternative-id":["10.1111\/nmo.12605"],"URL":"https:\/\/doi.org\/10.1111\/nmo.12605","archive":["Portico"],"relation":{},"ISSN":["1350-1925","1365-2982"],"issn-type":[{"value":"1350-1925","type":"print"},{"value":"1365-2982","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,31]]}}}