{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T06:31:22Z","timestamp":1787725882410,"version":"build-2784847793"},"reference-count":14,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,7,17]],"date-time":"2015-07-17T00:00:00Z","timestamp":1437091200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100014285","name":"Society of University Surgeons","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100014285","id-type":"DOI","asserted-by":"crossref"}]},{"name":"REACHirschsprung Foundation"},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["DK080914"],"award-info":[{"award-number":["DK080914"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Transplantation of enteric neural stem cells (<jats:styled-content style=\"fixed-case\">ENSC<\/jats:styled-content>) holds promise as a potential therapy for enteric neuropathies, including Hirschsprung disease. Delivery of transplantable cells via laparotomy has been described, but we propose a novel, minimally invasive endoscopic method of cell delivery.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Enteric neural stem cells for transplantation were cultured from dissociated gut of postnatal donor mice. Twelve recipient mice, including Ednrb<jats:sup>\u2212\/\u2212<\/jats:sup> mice with distal colonic aganglionosis, underwent colonoscopic injection of <jats:styled-content style=\"fixed-case\">ENSC<\/jats:styled-content> under direct vision using a 30\u2010gauge Hamilton needle passed through a rigid cystoureteroscope. Cell engraftment, survival, and neuroglial differentiation were studied 1\u20134\u00a0weeks after the procedure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>All recipient mice tolerated the procedure without complications and survived to sacrifice. Transplanted cells were found within the colonic wall in 9 of 12 recipient mice with differentiation into enteric neurons and glia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>Endoscopic injection of <jats:styled-content style=\"fixed-case\">ENSC<\/jats:styled-content> is a safe and reliable method for cell delivery, and can be used to deliver a large number of cells to a specific area of disease. This minimally invasive endoscopic approach may prove beneficial to future human applications of cell therapy for neurointestinal disease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12635","type":"journal-article","created":{"date-parts":[[2015,7,18]],"date-time":"2015-07-18T02:33:18Z","timestamp":1437186798000},"page":"1509-1514","source":"Crossref","is-referenced-by-count":34,"title":["Endoscopic delivery of enteric neural stem cells to treat Hirschsprung disease"],"prefix":"10.1111","volume":"27","author":[{"given":"L. S.","family":"Cheng","sequence":"first","affiliation":[{"name":"Department of Pediatric Surgery Massachusetts General Hospital Harvard Medical School Boston MA USA"},{"name":"Department of Surgery University of California San Francisco San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Hotta","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Massachusetts General Hospital Harvard Medical School Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. K.","family":"Graham","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Massachusetts General Hospital Harvard Medical School Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Nagy","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Massachusetts General Hospital Harvard Medical School Boston MA USA"},{"name":"Semmelweis University Budapest Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. M.","family":"Goldstein","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Massachusetts General Hospital Harvard Medical School Boston MA USA"},{"name":"Pediatric Neurogastroenterology Program MassGeneral Hospital for Children Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Belkind\u2010Gerson","sequence":"additional","affiliation":[{"name":"Pediatric Neurogastroenterology Program MassGeneral Hospital for Children Boston MA USA"},{"name":"Department of Pediatric Gastroenterology Massachusetts General Hospital Harvard Medical School Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,7,17]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2010.235614"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.sempedsurg.2009.07.008"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2006.094565"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.08.055"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2008.06.107"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2007.00981.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI65963"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.20095"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90017-5"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12015"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2009.06.038"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028858"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-964952"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.23.12846"}],"container-title":["Neurogastroenterology &amp; Motility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnmo.12635","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nmo.12635","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T19:28:37Z","timestamp":1696188517000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/nmo.12635"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7,17]]},"references-count":14,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2015,10]]}},"alternative-id":["10.1111\/nmo.12635"],"URL":"https:\/\/doi.org\/10.1111\/nmo.12635","archive":["Portico"],"relation":{},"ISSN":["1350-1925","1365-2982"],"issn-type":[{"value":"1350-1925","type":"print"},{"value":"1365-2982","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7,17]]}}}