{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T19:11:34Z","timestamp":1792005094743,"version":"4.4.0"},"reference-count":28,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,8,24]],"date-time":"2015-08-24T00:00:00Z","timestamp":1440374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003030","name":"Ag\u00e8ncia de Gesti\u00f3 d'Ajuts Universitaris i de Recerca","doi-asserted-by":"publisher","award":["2014 SGR 789"],"award-info":[{"award-number":["2014 SGR 789"]}],"id":[{"id":"10.13039\/501100003030","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Colonic samples from asymptomatic diverticulosis (<jats:styled-content style=\"fixed-case\">DS<\/jats:styled-content>) patients presented enhanced electrical field stimulation (<jats:styled-content style=\"fixed-case\">EFS<\/jats:styled-content>)\u2010contractions, in an earlier study of ours, suggesting increased endogenous responses. The aim of this study was to explore changes in excitatory neuromuscular transmission and to assess the pharmacodynamics of spasmolytic agents in <jats:styled-content style=\"fixed-case\">DS<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Circular muscle strips from\u00a0sigmoid colon of <jats:styled-content style=\"fixed-case\">DS<\/jats:styled-content> patients (<jats:italic>n<\/jats:italic>\u00a0=\u00a030; 69.5\u00a0\u00b1\u00a014.8\u00a0years) and controls (<jats:italic>n<\/jats:italic>\u00a0=\u00a032; 64.7\u00a0\u00b1\u00a016.2\u00a0years) were studied using organ baths to evaluate the direct effect of excitatory agonists (carbachol, neurokinin A [<jats:styled-content style=\"fixed-case\">NKA<\/jats:styled-content>] and substance P [<jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>]), and the effect of antagonists (atropine and <jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content><jats:sub>2<\/jats:sub> antagonist <jats:styled-content style=\"fixed-case\">GR<\/jats:styled-content>94800) and spasmolytic drugs (otilonium bromide [<jats:styled-content style=\"fixed-case\">OB<\/jats:styled-content>] and <jats:italic>N<\/jats:italic>\u2010butyl\u2010hyoscine) on the contractions induced by <jats:styled-content style=\"fixed-case\">EFS<\/jats:styled-content>\u2010stimulation of excitatory motorneurons. <jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> was also performed to compare <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression of M<jats:sub>2<\/jats:sub>, M<jats:sub>3<\/jats:sub>, <jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content><jats:sub>2<\/jats:sub> receptors and L\u2010type calcium channels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Contractions to carbachol (E<jats:sub>max<\/jats:sub>: 663.7\u00a0\u00b1\u00a0305.6% control <jats:italic>vs<\/jats:italic> 2698.0\u00a0\u00b1\u00a0439.5% <jats:styled-content style=\"fixed-case\">DS<\/jats:styled-content>;<jats:italic> p<\/jats:italic>\u00a0&lt;\u00a00.0005) and <jats:styled-content style=\"fixed-case\">NKA<\/jats:styled-content> (E<jats:sub>max<\/jats:sub>: 387.8\u00a0\u00b1\u00a035.6% <jats:italic>vs<\/jats:italic> 1102.0\u00a0\u00b1\u00a0190.1%; <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.0005) were higher in <jats:styled-content style=\"fixed-case\">DS<\/jats:styled-content> group, without differences for <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>. Higher potency for <jats:styled-content style=\"fixed-case\">DS<\/jats:styled-content> patients was observed in the concentration\u2010response curves for atropine (<jats:styled-content style=\"fixed-case\">pIC<\/jats:styled-content><jats:sub>50<\/jats:sub>\u00a0=\u00a08.56\u00a0\u00b1\u00a00.15 control <jats:italic>vs <\/jats:italic><jats:styled-content style=\"fixed-case\">pIC<\/jats:styled-content><jats:sub>50<\/jats:sub>\u00a0=\u00a09.95\u00a0\u00b1\u00a00.18 <jats:styled-content style=\"fixed-case\">DS<\/jats:styled-content> group; <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.005) and slightly higher for <jats:styled-content style=\"fixed-case\">GR<\/jats:styled-content>94800 (<jats:styled-content style=\"fixed-case\">pIC<\/jats:styled-content><jats:sub>50<\/jats:sub>\u00a0=\u00a07.21\u00a0\u00b1\u00a00.18 control <jats:italic>vs <\/jats:italic><jats:styled-content style=\"fixed-case\">pIC<\/jats:styled-content><jats:sub>50<\/jats:sub>\u00a0=\u00a07.97\u00a0\u00b1\u00a00.32 group; <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.0001). Lower efficacy (E<jats:sub>max<\/jats:sub>) and potency (<jats:styled-content style=\"fixed-case\">pIC<\/jats:styled-content><jats:sub>50<\/jats:sub>) was observed for spasmolytic drugs in <jats:styled-content style=\"fixed-case\">DS<\/jats:styled-content>, whereas no differences were found regarding the relative expression of the receptors evaluated between groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>The greater response to cholinergic and tachykinergic agonists and greater potency for muscarinic and <jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content><jats:sub>2<\/jats:sub> antagonists observed in <jats:styled-content style=\"fixed-case\">DS<\/jats:styled-content> might play a role in the spasticity found in diverticular disease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12659","type":"journal-article","created":{"date-parts":[[2015,8,25]],"date-time":"2015-08-25T04:10:24Z","timestamp":1440475824000},"page":"1600-1612","source":"Crossref","is-referenced-by-count":12,"title":["Changes in the response to excitatory antagonists, agonists, and spasmolytic agents in circular colonic smooth muscle strips from patients with diverticulosis"],"prefix":"10.1111","volume":"27","author":[{"given":"D.","family":"Alvarez\u2010Berdugo","sequence":"first","affiliation":[{"name":"Gastrointestinal Physiology Laboratory Department of Surgery Hospital de Matar\u00f3 Universitat Aut\u00f2noma de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Esp\u00edn","sequence":"additional","affiliation":[{"name":"Gastrointestinal Physiology Laboratory Department of Surgery Hospital de Matar\u00f3 Universitat Aut\u00f2noma de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Arenas","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de enfermedades hep\u00e1ticas y digestivas CIBERehd Instituto de Salud Carlos III Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"Gastrointestinal Physiology Laboratory Department of Surgery Hospital de Matar\u00f3 Universitat Aut\u00f2noma de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Clav\u00e9","sequence":"additional","affiliation":[{"name":"Gastrointestinal Physiology Laboratory Department of Surgery Hospital de Matar\u00f3 Universitat Aut\u00f2noma de Barcelona Barcelona Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de enfermedades hep\u00e1ticas y digestivas CIBERehd Instituto de Salud Carlos III Barcelona Spain"},{"name":"Health Sciences Research Institute of the Germans Trias i Pujol Foundation Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Gallego","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de enfermedades hep\u00e1ticas y digestivas CIBERehd Instituto de Salud Carlos III Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,8,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000099480"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/DCR.0b013e3181c18d64"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.32978"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v9.i10.2140"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.29.2.218"},{"key":"e_1_2_9_7_1","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1136\/gut.10.7.538","article-title":"Motility Studies in Diverticular Disease of Colon 2. Effect of Colonic and Rectal Distension","volume":"10","author":"Parks TG","year":"1969","journal-title":"Gut"},{"key":"e_1_2_9_8_1","first-page":"1163","article-title":"Colonic diverticular disease","volume":"84","author":"Humes D","year":"2011","journal-title":"Am Fam Physician"},{"key":"e_1_2_9_9_1","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1136\/gut.10.7.534","article-title":"Motility studies in diverticular disease of colon I. Basal activity and response to food assessed by open\u2010ended tube and miniature balloon techniques","volume":"10","author":"Parks TG","year":"1969","journal-title":"Gut"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/003655299750025886"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02050595"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02234460"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.102.034702"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.107.130385"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2011.01685.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)13583-0"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2004.01.014"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00384-013-1716-7"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2011.04586.x"},{"key":"e_1_2_9_20_1","first-page":"1009","article-title":"Demographic determinants of risk, colon distribution and density scores of diverticular disease","volume":"17","author":"Golder M","year":"2011","journal-title":"World J Gastroenterol"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjp.2008.332"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12407"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"e_1_2_9_24_1","first-page":"2839","article-title":"Physiological studies on nitric oxide in the right sided colon of patients with diverticular disease 4","volume":"46","author":"Tomita R","year":"1999","journal-title":"Hepatogastroenterology"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200767090-00007"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2010.01575.x"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2010.01602.x"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2010.01495.x"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2013.01.021"}],"container-title":["Neurogastroenterology &amp; Motility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnmo.12659","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nmo.12659","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T07:53:49Z","timestamp":1695542029000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/nmo.12659"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,8,24]]},"references-count":28,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2015,11]]}},"alternative-id":["10.1111\/nmo.12659"],"URL":"https:\/\/doi.org\/10.1111\/nmo.12659","archive":["Portico"],"relation":{},"ISSN":["1350-1925","1365-2982"],"issn-type":[{"value":"1350-1925","type":"print"},{"value":"1365-2982","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,8,24]]}}}