{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T04:35:10Z","timestamp":1791261310555,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,9,4]],"date-time":"2015-09-04T00:00:00Z","timestamp":1441324800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["G.0341.13"],"award-info":[{"award-number":["G.0341.13"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["G.0249.09N"],"award-info":[{"award-number":["G.0249.09N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2015,12]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>Visceral hypersensitivity is a main characteristic of functional bowel disorders and is mediated by both peripheral and central factors. We investigated whether enhanced splanchnic afferent signaling in\u00a0vitro<\/jats:italic> is associated with visceral hypersensitivity in\u00a0vivo<\/jats:italic> in an acute and postinflammatory rat model of colitis.<\/jats:p><\/jats:sec>Methods<\/jats:title>Trinitrobenzene sulfonic acid (TNBS<\/jats:styled-content>)\u2010colitis was monitored individually by colonoscopy to confirm colitis and follow convalescence and endoscopic healing in each rat. Experiments were performed in controls, rats with acute colitis and in postcolitis rats. Colonic afferent mechanosensitivity was assessed in\u00a0vivo<\/jats:italic> by quantifying visceromotor responses (VMR<\/jats:styled-content>s), and by making extracellular afferent recordings from splanchnic nerve bundles in\u00a0vitro<\/jats:italic>. Multiunit afferent activity was classified into single units identified as low threshold (LT<\/jats:styled-content>), wide dynamic range (WDR<\/jats:styled-content>), high threshold (HT<\/jats:styled-content>), and mechanically insensitive afferents (MIA<\/jats:styled-content>).<\/jats:p><\/jats:sec>Key Results<\/jats:title>During acute TNBS<\/jats:styled-content>\u2010colitis, VMR<\/jats:styled-content>s were significantly increased and splanchnic nerve recordings showed proportionally less MIA<\/jats:styled-content> and increased WDR<\/jats:styled-content> and HT<\/jats:styled-content> afferents. Acute colitis gave rise to an enhanced spontaneous activity of both LT<\/jats:styled-content> and MIA<\/jats:styled-content> and augmented afferent mechanosensitivity in LT<\/jats:styled-content>, WDR<\/jats:styled-content> and HT<\/jats:styled-content> afferents. Postcolitis, VMR<\/jats:styled-content>s remained significantly increased, whereas splanchnic nerve recordings showed that the proportion of LT<\/jats:styled-content>, WDR<\/jats:styled-content>, HT<\/jats:styled-content> and MIA<\/jats:styled-content> had normalized to control values. However, LT<\/jats:styled-content> and MIA<\/jats:styled-content> continued to show increased spontaneous activity and WDR<\/jats:styled-content> and HT<\/jats:styled-content> remained sensitized to colorectal distension.<\/jats:p><\/jats:sec>Conclusions & Inferences<\/jats:title>Visceral hypersensitivity in\u00a0vivo<\/jats:italic> is associated with sensitized splanchnic afferent responses both during acute colitis and in the postinflammatory phase. However, splanchnic afferent subpopulations are affected differentially at both time points.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12667","type":"journal-article","created":{"date-parts":[[2015,9,4]],"date-time":"2015-09-04T11:39:45Z","timestamp":1441366785000},"page":"1709-1720","source":"Crossref","is-referenced-by-count":12,"title":["Mechanisms contributing to visceral hypersensitivity: focus on splanchnic afferent nerve signaling"],"prefix":"10.1111","volume":"27","author":[{"given":"A.","family":"Deiteren","sequence":"first","affiliation":[{"name":"Laboratory of Experimental Medicine and Pediatrics Division of Gastroenterology University of Antwerp Antwerp Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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