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In this study, we investigated whether a<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010like degeneration of dopaminergic neurons of the substantia nigra can influence peristalsis in colonic segments of rats by impacting on enteric dopaminergic transmission.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Male, Sprague\u2013Dawley rats received a unilateral injection of neurotoxin 6\u2010hydroxydopamine (6\u2010<jats:styled-content style=\"fixed-case\">OHDA<\/jats:styled-content>), or saline, into the medial\u2010forebrain\u2010bundle. Peristaltic activity was recorded in isolated colonic segments, in baseline conditions and following exposure to combinations of D2 receptor (<jats:styled-content style=\"fixed-case\">DRD<\/jats:styled-content>2) agonist sumanirole and antagonist L\u2010741626. Dopamine levels and<jats:styled-content style=\"fixed-case\">DRD<\/jats:styled-content>2 expression were assessed in the ileum and colon of animals. We also investigated the involvement of the dorsal motor nucleus of the vagus (<jats:styled-content style=\"fixed-case\">DMV<\/jats:styled-content>) \u2014 a potential relay station between central dopaminergic denervation and gastrointestinal (<jats:styled-content style=\"fixed-case\">GI<\/jats:styled-content>) dysfunction \u2014 by analyzing cytochrome c oxidase activity and FosB\/DeltaFosB expression in<jats:styled-content style=\"fixed-case\">DMV<\/jats:styled-content>neurons.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>We observed profound alterations in the response of colonic segments of 6\u2010<jats:styled-content style=\"fixed-case\">OHDA<\/jats:styled-content>lesioned animals to<jats:styled-content style=\"fixed-case\">DRD<\/jats:styled-content>2 stimulation. In fact, the inhibition of colonic peristalsis elicited by sumanirole in control rats was absent in 6\u2010<jats:styled-content style=\"fixed-case\">OHDA<\/jats:styled-content>\u2010lesioned animals. These animals also showed reduced<jats:styled-content style=\"fixed-case\">DRD<\/jats:styled-content>2 expression in the colon, along with elevation of dopamine levels. No significant changes were detected within the<jats:styled-content style=\"fixed-case\">DMV<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>Our results demonstrate that selective lesion of the nigrostriatal dopaminergic pathway subverts the physiological response of the colon to dopaminergic stimulation, opening new perspectives in the comprehension and treatment of<jats:styled-content style=\"fixed-case\">GI<\/jats:styled-content>dysfunctions associated with<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12691","type":"journal-article","created":{"date-parts":[[2015,10,3]],"date-time":"2015-10-03T13:34:59Z","timestamp":1443879299000},"page":"1783-1795","source":"Crossref","is-referenced-by-count":22,"title":["Response of colonic motility to dopaminergic stimulation is subverted in rats with nigrostriatal lesion: relevance to gastrointestinal dysfunctions in Parkinson's disease"],"prefix":"10.1111","volume":"27","author":[{"given":"G.","family":"Levandis","sequence":"first","affiliation":[{"name":"Laboratory of Functional Neurochemistry Center for Research in Neurodegenerative Diseases \u201cC. 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