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Amongst these, radiographic techniques after contrast intragastric administration may offer many advantages. In previous studies, we have successfully and reproducibly applied these techniques together with a semiquantitative analysis method to characterize the effect of different drugs, acutely or repeatedly administered in rat models, but we have never before used these techniques in mice. These are very convenient in basic research. Our aim was to determine if our method is also valid in mice. Additionally, we determined the effect of morphine on<jats:styled-content style=\"fixed-case\">GI<\/jats:styled-content>motor function in both species.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Animals received an intraperitoneal administration of morphine (at 10 and 5 mg\/kg for rats and mice, respectively). Twenty min later, barium contrast (at 2 g\/mL) was gavaged (2.5 and 0.4 mL for rats and mice respectively) and serial X\u2010rays were obtained 0\u20138 h after contrast. X\u2010rays were analyzed as previously described, using a semiquantitative score to build motility curves for each<jats:styled-content style=\"fixed-case\">GI<\/jats:styled-content>region.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Motility was much faster in mice than in rats for all<jats:styled-content style=\"fixed-case\">GI<\/jats:styled-content>regions. Morphine at the doses used significantly depressed motility in both species to a similar extent if the whole gut or the upper<jats:styled-content style=\"fixed-case\">GI<\/jats:styled-content>regions (stomach, small intestine) were considered, although its effect seemed to be more intense in the lower<jats:styled-content style=\"fixed-case\">GI<\/jats:styled-content>regions (caecum, colorectum) in rats than in mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>We have validated our X\u2010rays method for its use in mice.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12699","type":"journal-article","created":{"date-parts":[[2015,10,22]],"date-time":"2015-10-22T03:00:05Z","timestamp":1445482805000},"page":"74-84","source":"Crossref","is-referenced-by-count":21,"title":["X\u2010ray analysis of gastrointestinal motility in conscious mice. 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Investigaciones Cient\u00edficas (CSIC) Grupo de Excelencia Investigadora URJC\u2010Banco de Santander\u2010Grupo Multidisciplinar de Investigaci\u00f3n y Tratamiento del Dolor (i+DOL) Universidad Rey Juan Carlos Alcorc\u00f3n Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Abalo","sequence":"additional","affiliation":[{"name":"\u00c1rea de Farmacolog\u00eda y Nutrici\u00f3n y Unidad Asociada al Instituto de Qu\u00edmica M\u00e9dica (IQM) y al Instituto de Investigaci\u00f3n en Ciencias de la Alimentaci\u00f3n (CIAL) del Consejo Superior de Investigaciones Cient\u00edficas (CSIC) Grupo de Excelencia Investigadora URJC\u2010Banco de Santander\u2010Grupo Multidisciplinar de Investigaci\u00f3n y Tratamiento del Dolor (i+DOL) Universidad Rey Juan Carlos Alcorc\u00f3n Madrid 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