{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:36:04Z","timestamp":1791351364379,"version":"4.3.1"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,10,28]],"date-time":"2015-10-28T00:00:00Z","timestamp":1445990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s) are known to be involved in normal brain functions and nervous system diseases. Some evidence have pointed to the dysregulation of mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s in congenital intestinal atresia. In this study, we investigated the differential expression of mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s and the posttranscriptional regulation of glial\u2010derived neurotrophic factor (<jats:styled-content style=\"fixed-case\">GDNF<\/jats:styled-content>) by endogenous mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> in congenital intestinal atresia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Quantitative real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and a Western blot were performed to determine the regulation of mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">GDNF<\/jats:styled-content> in patients with congenital intestinal atresia. The results were verified in rat model of intestinal atresia and bone marrow derived stem cell <jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s\u2010derived into intestinal ganglion cells. The effects of mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">GDNF<\/jats:styled-content> on the cell proliferation and apoptosis of isolated intestinal ganglion cells were detected with an 3\u2010(4,5\u2010dimethylthiazol)\u20102,5\u2010diphenyl tetrazolium (<jats:styled-content style=\"fixed-case\">MTT<\/jats:styled-content>) assay and a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Only miR\u2010211 was greatly up\u2010regulated in the patients with congenital intestinal atresia. The other mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s examined showed no change. Overexpression of miR\u2010211 suppressed the differentiation of <jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s into intestinal ganglion cells. In retinal ganglion cells (<jats:styled-content style=\"fixed-case\">RGC<\/jats:styled-content>\u20105 cells), miR\u2010211 regulated the expression of <jats:styled-content style=\"fixed-case\">GDNF<\/jats:styled-content>. The <jats:styled-content style=\"fixed-case\">MTT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">TUNEL<\/jats:styled-content> assays revealed that miR\u2010211 overexpression suppressed the cell proliferation of isolated intestinal ganglion cells and that <jats:styled-content style=\"fixed-case\">GDNF<\/jats:styled-content> overexpression reversed the effect of pre\u2010miR\u2010211 on cell proliferation and apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>Our results indicate that overexpression of miR\u2010211 suppresses the differentiation of <jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>s into intestinal ganglion cells by directly down\u2010regulating the expression of <jats:styled-content style=\"fixed-case\">GDNF<\/jats:styled-content>. The findings elucidate the role of mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> in congenital intestinal atresia.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12705","type":"journal-article","created":{"date-parts":[[2015,10,29]],"date-time":"2015-10-29T04:10:16Z","timestamp":1446091816000},"page":"186-195","source":"Crossref","is-referenced-by-count":12,"title":["Micro<scp>RNA<\/scp>\u2010211 causes ganglion cell dysplasia in congenital intestinal atresia via down\u2010regulation of glial\u2010derived neurotrophic factor"],"prefix":"10.1111","volume":"28","author":[{"given":"Z.\u2010q.","family":"Xia","sequence":"first","affiliation":[{"name":"Department of Pediatric Surgery The First Affiliated Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.\u2010k.","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery The First Affiliated Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery The First Affiliated Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.\u2010x.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery The First Affiliated Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.\u2010y.","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery The First Affiliated Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery The First Affiliated Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,10,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1886"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199604253341707"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/jpsu.2001.21618"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neures.2013.08.012"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2936"},{"key":"e_1_2_9_7_1","first-page":"768","volume-title":"Seminars in Cell & Developmental Biology","author":"Lau P","year":"2010"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuro.2012.02.013"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004410050867"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2000.0752227.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.1999.0754"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn812"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.12.2785"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2006.07.019"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0062292"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11596-012-0015-9"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2011.08689.x"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2014.06.002"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2013.05.025"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2009.06.009"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3468(99)90104-5"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1203\/01.PDR.0000145294.11800.71"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0013779"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0049145"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2013.05.032"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2014.03.010"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0029750"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(03)00404-9"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2014.01.008"}],"container-title":["Neurogastroenterology &amp; 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