{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T23:12:09Z","timestamp":1788649929688,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,11,6]],"date-time":"2015-11-06T00:00:00Z","timestamp":1446768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004434","name":"University of Florence","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004434","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Glucagon\u2010like peptide\u20102 (<jats:styled-content style=\"fixed-case\">GLP<\/jats:styled-content>\u20102) is a pleiotropic hormone synthesized and secreted by the enteroendocrine \u2018L\u2019 cells able to exert intestine\u2010trophic and anti\u2010inflammatory effects. The antineoplastic drug cisplatin causes gastrointestinal alterations with clinical symptoms (nausea and vomiting) that greatly affect the therapy compliance. Experimentally, it has been reported that chronic cisplatin treatment caused mucosal damage and enteric neuropathy in the rat colon.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We investigated, through a combined immunohistochemical and functional approach, whether [Gly<jats:sup>2<\/jats:sup>]<jats:styled-content style=\"fixed-case\">GLP<\/jats:styled-content>\u20102, a <jats:styled-content style=\"fixed-case\">GLP<\/jats:styled-content>\u20102 analog, was able to counteract the detrimental effects of long\u2010term cisplatin administration in the mucosa and myenteric neurons of mouse gastric fundus.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Morphological experiments showed a reduction in the epithelium thickness in cisplatin\u2010treated mice, which was prevented by [Gly<jats:sup>2<\/jats:sup>]<jats:styled-content style=\"fixed-case\">GLP<\/jats:styled-content>\u20102 co\u2010treatment. Immunohistochemistry demonstrated that cisplatin caused a significant decrease in myenteric neurons, mainly those expressing neuronal nitric oxide synthase (<jats:styled-content style=\"fixed-case\">nNOS<\/jats:styled-content>), that was prevented by [Gly<jats:sup>2<\/jats:sup>]<jats:styled-content style=\"fixed-case\">GLP<\/jats:styled-content>\u20102 co\u2010treatment. In the functional experiments, [Gly<jats:sup>2<\/jats:sup>]<jats:styled-content style=\"fixed-case\">GLP<\/jats:styled-content>\u20102 co\u2010treatment counteracted the increase in amplitude of the neurally induced contractions observed in strips from cisplatin\u2010treated animals. The <jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content> synthesis inhibitor L\u2010N<jats:sup>G<\/jats:sup>\u2010nitro arginine caused an increase in amplitude of the contractile responses that was greater in preparations from cisplatin+[Gly<jats:sup>2<\/jats:sup>]<jats:styled-content style=\"fixed-case\">GLP<\/jats:styled-content>\u20102 treated mice compared to the cisplatin\u2010treated ones.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>The results demonstrate that in cisplatin long\u2010term treated mice [Gly<jats:sup>2<\/jats:sup>]<jats:styled-content style=\"fixed-case\">GLP<\/jats:styled-content>\u20102 is able to counteract both the mucosal gastric fundus damage, by preventing the epithelium thickness decrease, and the neuropathy, by protecting the <jats:styled-content style=\"fixed-case\">nNOS<\/jats:styled-content> neurons. Taken together, the present data suggest that [Gly<jats:sup>2<\/jats:sup>]<jats:styled-content style=\"fixed-case\">GLP<\/jats:styled-content>\u20102 could represent an effective strategy to overcome the distressing gastrointestinal symptoms present during the anti\u2010neoplastic therapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12712","type":"journal-article","created":{"date-parts":[[2015,11,8]],"date-time":"2015-11-08T19:37:51Z","timestamp":1447011471000},"page":"206-216","source":"Crossref","is-referenced-by-count":42,"title":["Glucagon\u2010like peptide 2 counteracts the mucosal damage and the neuropathy induced by chronic treatment with cisplatin in the mouse gastric fundus"],"prefix":"10.1111","volume":"28","author":[{"given":"A.","family":"Pini","sequence":"first","affiliation":[{"name":"Department of Experimental and Clinical Medicine Histology and Embryology Research Unit University of Florence Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Garella","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine Section of Physiological Sciences University of Florence Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Idrizaj","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine Section of Physiological Sciences University of Florence Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Calosi","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine Histology and Embryology Research Unit University of Florence Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. C.","family":"Baccari","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine Section of Physiological Sciences University of Florence Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. G.","family":"Vannucchi","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine Histology and Embryology Research Unit University of Florence Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,11,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2013.01.014"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2002-0306"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.15.7911"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1997.273.1.E77"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-12-0500"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1517\/14728222.2011.556620"},{"key":"e_1_2_10_8_1","first-page":"12","article-title":"Glucagon\u2010like paptides 1 and 2 and vasoactive intestinal peptide are neuroprotective on cultured and mast cell co\u2010cultured rat myenteric neurons","volume":"1","author":"Voss U","year":"2012","journal-title":"BMC Gastroenterol"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-009-1104-x"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2011.01750.x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00039.2011"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.4.1569"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.regpep.2004.07.009"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1517\/13543784.16.7.1009"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2014.07.025"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114511007239"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpg.2008.11.016"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2010.01483.x"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autneu.2006.01.008"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002800050581"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2011.01674.x"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2004.061440"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.regpep.2009.12.012"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00059.2013"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00375.2010"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autneu.2008.05.004"},{"key":"e_1_2_10_27_1","first-page":"647","article-title":"Neuropharmacological mechanisms of emesis. II. Effects of antiemetic drugs on cisplatin\u2010induced pica in rats","volume":"17","author":"Takeda N","year":"1995","journal-title":"Methods Find Exp Clin Pharmacol"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1997.272.6.E1050"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00280-012-1882-2"},{"key":"e_1_2_10_30_1","first-page":"616054","article-title":"Short bowel patients treated for two years with glucagon\u2010like peptide 2: effects on intestinal morphology and absorption, renal function, bone and body composition, and muscle function","volume":"2009","author":"Jeppesen PB","year":"2009","journal-title":"Gastroenterol Res Pract"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2013.00030"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4886(03)00003-7"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00212.2014"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/nos203"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(92)90707-B"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(95)90054-3"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6604375"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12061"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.02.033"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOG.0000000000000046"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1097\/MED.0000000000000120"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2003.035212"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00505.2011"}],"container-title":["Neurogastroenterology &amp; Motility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnmo.12712","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nmo.12712","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T17:54:53Z","timestamp":1696182893000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/nmo.12712"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11,6]]},"references-count":42,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,2]]}},"alternative-id":["10.1111\/nmo.12712"],"URL":"https:\/\/doi.org\/10.1111\/nmo.12712","archive":["Portico"],"relation":{},"ISSN":["1350-1925","1365-2982"],"issn-type":[{"value":"1350-1925","type":"print"},{"value":"1365-2982","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,11,6]]}}}