{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:06:23Z","timestamp":1792677983881,"version":"4.5.0"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,11,17]],"date-time":"2015-11-17T00:00:00Z","timestamp":1447718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81370473"],"award-info":[{"award-number":["81370473"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81400574"],"award-info":[{"award-number":["81400574"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20131388"],"award-info":[{"award-number":["BK20131388"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hirschsprung's disease (<jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>) is one of the common digestive disorders in the new born. Long non\u2010coding <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s) play an important role in various biological processes. However, knowledge on lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s in <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> is limited.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The expression profile of lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s in <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> was obtained using microarray. A total of 2078 differentially expressed lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were detected by microarray in <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> tissues compared with matched normal colon tissues (fold change \u22652, <jats:italic>p<\/jats:italic> &lt; 0.05). Candidate biomarkers were selected from these differentially expressed lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s based on artificial criterion (raw signal intensity \u226550; fold change \u22658) and then validated in 80 pairs of <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> and normal tissues using quantitative real\u2010time polymerase chain reaction (<jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>). Moreover, the computational analysis was used to evaluate the lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> and lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2013protein relationships.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>A panel of 5\u2010lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s was identified to distinguish <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> from normal tissues with remarkable sensitivity and specificity. The area under the receiver operating characteristic curve (<jats:styled-content style=\"fixed-case\">AUC<\/jats:styled-content>) for <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> identification in the validation set was 0.875. The bioinformatics analysis reveals that these dysregulated lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s are mainly involved in <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2013protein relationships, including <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> splicing, binding, transport, processing, and localization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>Our results are the first to report the expression profile of dysregulated lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s in <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> and infer that lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s may serve as novel diagnostic biomarkers for <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12722","type":"journal-article","created":{"date-parts":[[2015,11,17]],"date-time":"2015-11-17T23:42:43Z","timestamp":1447803763000},"page":"266-273","source":"Crossref","is-referenced-by-count":19,"title":["Microarray expression profiling of dysregulated long non\u2010coding <scp>RNA<\/scp>s in Hirschsprung's disease reveals their potential role in molecular diagnosis"],"prefix":"10.1111","volume":"28","author":[{"given":"Z.","family":"Shen","sequence":"first","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine Institute of Toxicology School of Public Health Nanjing Medical University Nanjing China"},{"name":"Department of Pediatric Surgery Nanjing Children's Hospital Affiliated Nanjing Medical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Du","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine Institute of Toxicology School of Public Health Nanjing Medical University Nanjing China"},{"name":"Department of Pediatric Surgery Nanjing Children's Hospital Affiliated Nanjing Medical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Zang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine Institute of Toxicology School of Public Health Nanjing Medical University Nanjing China"},{"name":"Department of Pediatric Surgery Nanjing Children's Hospital Affiliated Nanjing Medical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Xie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine Institute of Toxicology School of Public Health Nanjing Medical University Nanjing China"},{"name":"Department of Pediatric Surgery Nanjing Children's Hospital Affiliated Nanjing Medical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Accounting School of Business Nanjing University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine Institute of Toxicology School of Public Health Nanjing Medical University Nanjing China"},{"name":"Department of Pediatric Surgery Nanjing Children's Hospital Affiliated Nanjing Medical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Xia","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine Institute of Toxicology School of Public Health Nanjing Medical University Nanjing China"},{"name":"Key Laboratory of Modern Toxicology (Nanjing Medical University) Ministry of Education Nanjing 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