{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T06:40:21Z","timestamp":1784356821818,"version":"3.55.0"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,1,6]],"date-time":"2016-01-06T00:00:00Z","timestamp":1452038400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>A relationship between stress and the symptoms of irritable bowel syndrome (<jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content>) has been well established but the cellular mechanisms are poorly understood. Therefore, we investigated effects of stress and stress hormones on colonic descending inhibition and transit in mouse models and human tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Stress was applied using water avoidance stress (<jats:styled-content style=\"fixed-case\">WAS<\/jats:styled-content>) in the animal model or mimicked using stress hormones, adrenaline (5 nM), and corticosterone (1 <jats:italic>\u03bc<\/jats:italic>M). Intracellular recordings were obtained from colonic circular smooth muscle cells in isolated smooth muscle\/myenteric plexus preparations and the inhibitory junction potential (<jats:styled-content style=\"fixed-case\">IJP<\/jats:styled-content>) was elicited by nerve stimulation or balloon distension oral to the site of recording.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Water avoidance stress increased the number of fecal pellets compared to control (<jats:italic>p<\/jats:italic> &lt; 0.05). <jats:styled-content style=\"fixed-case\">WAS<\/jats:styled-content> also caused a significant increase in <jats:styled-content style=\"fixed-case\">IJP<\/jats:styled-content> amplitude following balloon distension. Stress hormones also increased the <jats:styled-content style=\"fixed-case\">IJP<\/jats:styled-content> amplitude following nerve stimulation and balloon distension (<jats:italic>p<\/jats:italic> &lt; 0.05) in control mice but had no effect in colons from stressed mice. No differences were observed with application of <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content> between stress and control tissues, suggesting the actions of stress hormones were presynaptic. Stress hormones had a large effect in the nerve stimulated <jats:styled-content style=\"fixed-case\">IJP<\/jats:styled-content> in human colon (increased &gt;50%). Immunohistochemical studies identified alpha and beta adrenergic receptor immunoreactivity on myenteric neurons in human colon.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>These studies suggest that <jats:styled-content style=\"fixed-case\">WAS<\/jats:styled-content> and stress hormones can signal via myenteric neurons to increase inhibitory neuromuscular transmission. This could lead to greater descending relaxation, decreased transit time, and subsequent diarrhea.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12755","type":"journal-article","created":{"date-parts":[[2016,1,8]],"date-time":"2016-01-08T05:28:55Z","timestamp":1452230935000},"page":"569-580","source":"Crossref","is-referenced-by-count":15,"title":["Stress increases descending inhibition in mouse and human colon"],"prefix":"10.1111","volume":"28","author":[{"given":"D. E.","family":"Reed","sequence":"first","affiliation":[{"name":"GI Diseases Research Unit Queen's University Kingston ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Zhang","sequence":"additional","affiliation":[{"name":"GI Diseases Research Unit Queen's University Kingston ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. J.","family":"Beyak","sequence":"additional","affiliation":[{"name":"GI Diseases Research Unit Queen's University Kingston ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Lourenssen","sequence":"additional","affiliation":[{"name":"GI Diseases Research Unit Queen's University Kingston ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. G.","family":"Blennerhassett","sequence":"additional","affiliation":[{"name":"GI Diseases Research Unit Queen's University Kingston ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. G.","family":"Paterson","sequence":"additional","affiliation":[{"name":"GI Diseases Research Unit Queen's University Kingston ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. J.","family":"Vanner","sequence":"additional","affiliation":[{"name":"GI Diseases Research Unit Queen's University Kingston ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,1,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.37095"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.01.032"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)90341-4"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.44.3.400"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2009.02.074"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2001.280.1.G7"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00500.2004"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.1998.00293.x"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(87)90319-2"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(95)90743-2"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2001.280.2.G173"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.90712.2008"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.01.039"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(91)90270-U"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.059659"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00713.2005"},{"issue":"4","key":"e_1_2_11_18_1","first-page":"G582","article-title":"Corticotropin\u2010releasing factor directly mediates colonic responses to stress","volume":"253","author":"Williams CL","year":"1987","journal-title":"Am J Physiol"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.00465.x"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1838(91)90229-V"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12268"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00052.2002"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.0235o.x"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00266.2009"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.109.160978"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00448.2009"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.08.006"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00510.2007"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00474.2005"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(93)90943-7"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12802"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2009.185082"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2011.224634"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2005.083600"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20898"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902040411"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1999.81.3.1104"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn3138"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2010.01516.x"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.43.2.256"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2009.239"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(88)90006-6"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00011.2009"}],"container-title":["Neurogastroenterology &amp; Motility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnmo.12755","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnmo.12755","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nmo.12755","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T14:42:14Z","timestamp":1695480134000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/nmo.12755"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1,6]]},"references-count":42,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2016,4]]}},"alternative-id":["10.1111\/nmo.12755"],"URL":"https:\/\/doi.org\/10.1111\/nmo.12755","archive":["Portico"],"relation":{},"ISSN":["1350-1925","1365-2982"],"issn-type":[{"value":"1350-1925","type":"print"},{"value":"1365-2982","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1,6]]}}}