{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T11:22:20Z","timestamp":1780917740538,"version":"3.54.1"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,1,27]],"date-time":"2016-01-27T00:00:00Z","timestamp":1453852800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009108","name":"Shandong University","doi-asserted-by":"publisher","award":["2014QLKY08"],"award-info":[{"award-number":["2014QLKY08"]}],"id":[{"id":"10.13039\/100009108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) is endogenously generated from L\u2010cysteine (L\u2010Cys) by the enzymes cystathionine\u2010<jats:italic>\u03b2<\/jats:italic>\u2010synthase (<jats:styled-content style=\"fixed-case\">CBS<\/jats:styled-content>) and cystathionine\u2010<jats:italic>\u03b3<\/jats:italic>\u2010Lyase (<jats:styled-content style=\"fixed-case\">CSE<\/jats:styled-content>). Hydrogen sulfide is also produced from D\u2010cysteine (D\u2010Cys) by D\u2010Amino acid oxidase (<jats:styled-content style=\"fixed-case\">DAO<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The H<jats:sub>2<\/jats:sub>S production was measured by the methylene blue assay. The expression of <jats:styled-content style=\"fixed-case\">DAO<\/jats:styled-content> was investigated by Western blotting and immunohistochemistry. The short\u2010circuit current (<jats:italic>I<\/jats:italic>sc) was recorded using the Ussing chamber technique.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>The epithelium in rat jejunum possesses <jats:styled-content style=\"fixed-case\">DAO<\/jats:styled-content>, and generates H<jats:sub>2<\/jats:sub>S. D\u2010cysteine, originally used as a negative control for L\u2010Cys, significantly increases the H<jats:sub>2<\/jats:sub>S release, which is inhibited by I2<jats:styled-content style=\"fixed-case\">CA<\/jats:styled-content>, an inhibitor of <jats:styled-content style=\"fixed-case\">DAO<\/jats:styled-content>. <jats:italic>In vitro<\/jats:italic> study by Ussing chamber technique reveals that D\u2010Cys decreases the <jats:italic>I<\/jats:italic>sc across the epithelium of the rat jejunum and enhances the Na<jats:sup>+<\/jats:sup>\u2010coupled L\u2010alanine transport.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>A novel pathway for the production of H<jats:sub>2<\/jats:sub>S by <jats:styled-content style=\"fixed-case\">DAO<\/jats:styled-content> exists in rat jejunum.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12765","type":"journal-article","created":{"date-parts":[[2016,1,27]],"date-time":"2016-01-27T05:58:43Z","timestamp":1453874323000},"page":"687-692","source":"Crossref","is-referenced-by-count":12,"title":["A novel pathway for the production of H<sub>2<\/sub>S by <scp>DAO<\/scp> in rat jejunum"],"prefix":"10.1111","volume":"28","author":[{"given":"S.","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Qilu Hospital Shandong University Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Medicine Shandong University Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"An","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Medicine Shandong University Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Medicine Shandong University Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery Shandong Tumor Hospital Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,1,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-03-01066.1996"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6878"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2060267"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.010868"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20110841"},{"key":"e_1_2_8_7_1","first-page":"483","article-title":"Purification and characterization of mitochondrial cysteine aminotransferase from rat liver","volume":"10","author":"Ubuka T","year":"1978","journal-title":"Physiol Chem Phys"},{"key":"e_1_2_8_8_1","first-page":"187","article-title":"Purification and characterization of cysteine aminotransferase from rat liver cytosol","volume":"36","author":"Akagi R","year":"1982","journal-title":"Acta Med Okayama"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.52.070183.001155"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2371"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2008.2253"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.107.3.897"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.298208"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20040243"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvp111"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0914128107"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2011.01.026"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2009.05.016"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2013.5312"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2006.08.035"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12562"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(81)90139-2"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2185543"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00971320"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2009.00385.x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/00075197-200201000-00014"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1998.78.4.969"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(96)00683-8"}],"container-title":["Neurogastroenterology &amp; 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