{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T10:45:58Z","timestamp":1781174758214,"version":"3.54.1"},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,1,25]],"date-time":"2016-01-25T00:00:00Z","timestamp":1453680000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hirschsprung's disease (<jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>), a birth defect characterized by variable aganglionosis of the gut, affects about 1 in 5000 births and is a consequence of abnormal development of neural crest cells, from which enteric ganglia derive. In the companion article in this issue (Shen <jats:italic>et\u00a0al<\/jats:italic>., Neurogasterenterol Motil 28: 266\u201373), the authors search for long non\u2010coding <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s) differentially expressed in bowel tissues of infants with <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>. Microarray analysis of over 37\u00a0000 lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s and 34\u00a0000 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>s was done. The key result was identification of a set of 5 lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s that is a potential diagnostic biomarker of <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>. In this minireview, I provide an overview of neural crest development and the gene regulatory networks involved in specification, epithelial\u2013mesenchymal transition, and migration of neural crest cells. Genes involved in later development, proliferation, and differentiation of neural crest cells as they migrate into the gut are also reviewed. Many of these genes are associated with <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>, including <jats:styled-content style=\"fixed-case\">RET<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> GDNF<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> GFR<\/jats:styled-content><jats:italic>\u03b1<\/jats:italic>,<jats:styled-content style=\"fixed-case\"> EDN<\/jats:styled-content>3, and <jats:styled-content style=\"fixed-case\">EDNRB<\/jats:styled-content>. Lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s and their roles in development and disease and their use as biomarkers are discussed. The authors of the companion article previously used a multipronged approach to elucidate the etiology of <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> by examining the effects of specific mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s or lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s and target genes on cell migration, proliferation, cell cycle, and apoptosis <jats:italic>in\u00a0vitro<\/jats:italic>. These studies are discussed in terms of their elegance and limitations. The companion article identifies many new lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s that, in addition to providing potential biomarkers of <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>, may be a treasure trove for future investigations.<\/jats:p>","DOI":"10.1111\/nmo.12776","type":"journal-article","created":{"date-parts":[[2016,1,25]],"date-time":"2016-01-25T07:29:39Z","timestamp":1453706979000},"page":"161-166","source":"Crossref","is-referenced-by-count":12,"title":["Search for the missing lncs: gene regulatory networks in neural crest development and long non\u2010coding <scp>RNA<\/scp> biomarkers of Hirschsprung's disease"],"prefix":"10.1111","volume":"28","author":[{"given":"J. M.","family":"Rogers","sequence":"first","affiliation":[{"name":"Toxicity Assessment Division National Health and Environmental Effects Research Laboratory Office of Research and Development United States Environmental Protection Agency Research Triangle Park NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,1,25]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.38.11.729"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12722"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/cge.12054"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.105445"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.161182.113"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2013.03.001"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2013.05.019"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00383-015-3747-3"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/3984937"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12625"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2013.01.022"},{"key":"e_1_2_11_13_1","first-page":"S1874\u20109399(15)0","article-title":"Dynamic interplay and function of multiple noncoding genes governing X chromosome inactivation","author":"Yue M","year":"2015","journal-title":"Biochim Biophys Acta"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmt117"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.304156"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.22581"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.02.012"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers7040884"},{"key":"e_1_2_11_19_1","first-page":"3966","article-title":"Expression profiles of HA117 and its neighboring gene DPF3 in different colon segments of Hirschprung's disease","volume":"7","author":"Liu H","year":"2014","journal-title":"Int J Clin Exp Pathol"},{"key":"e_1_2_11_20_1","first-page":"3979","article-title":"Differential expression of FOXA1, DUSP6, and HA117 in colon segments of Hirschsprung's disease","volume":"8","author":"Luo Y","year":"2015","journal-title":"Int J Clin Exp Pathol"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12348"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-230X-14-123"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep09302"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.13118"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1159\/000356601"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1159\/000363021"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12454"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.05.096"}],"container-title":["Neurogastroenterology &amp; Motility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnmo.12776","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nmo.12776","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T17:55:36Z","timestamp":1696182936000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/nmo.12776"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1,25]]},"references-count":27,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,2]]}},"alternative-id":["10.1111\/nmo.12776"],"URL":"https:\/\/doi.org\/10.1111\/nmo.12776","archive":["Portico"],"relation":{},"ISSN":["1350-1925","1365-2982"],"issn-type":[{"value":"1350-1925","type":"print"},{"value":"1365-2982","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1,25]]}}}