{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T09:37:49Z","timestamp":1790674669288,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,2,22]],"date-time":"2016-02-22T00:00:00Z","timestamp":1456099200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Australian National Health & Medical Research Council Project","award":["1032414"],"award-info":[{"award-number":["1032414"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Patients receiving anticancer chemotherapy experience a multitude of gastrointestinal side\u2010effects. However, the causes of these symptoms are uncertain and whether these therapeutics directly affect the enteric nervous system is unknown. Our aim was to determine whether the function and morphology of myenteric neurons are altered in specimens of the colon from chemotherapy\u2010treated patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Colon specimens were compared from chemotherapy\u2010treated and non\u2010treated patients following colorectal resections for removal of carcinoma. Intracellular electrophysiological recordings from myenteric neurons and immunohistochemistry were performed in whole mount preparations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Myenteric <jats:italic>S<\/jats:italic> neurons from chemotherapy\u2010treated patients were hyperexcitable; more action potentials (11.4 \u00b1 9.4, <jats:italic>p<\/jats:italic> &lt; 0.05) were fired in response to depolarising current pulses than in non\u2010treated patients (1.4 \u00b1 0.5). The rheobase and the threshold to evoke action potentials were significantly lower for neurons from chemotherapy\u2010treated patients compared to neurons from non\u2010treated patients (<jats:italic>p<\/jats:italic> &lt; 0.01). Fast excitatory postsynaptic potential reversal potential was more positive in neurons from chemotherapy\u2010treated patients (<jats:italic>p<\/jats:italic> &lt; 0.05). An increase in the number of neurons with translocation of Hu protein from the cytoplasm to the nucleus was observed in specimens from chemotherapy\u2010treated patients (103 \u00b1 25 neurons\/mm<jats:sup>2<\/jats:sup>, 37.2 \u00b1 7.0%, <jats:italic>n<\/jats:italic> = 8) compared to non\u2010treated (26 \u00b1 5 neurons\/mm<jats:sup>2<\/jats:sup>, 11.9 \u00b1 2.7%, <jats:italic>n<\/jats:italic> = 12, <jats:italic>p<\/jats:italic> &lt; 0.01). An increase in the soma size of neuronal nitric oxide synthase\u2010immunoreactive neurons was also observed in these specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>This is the first study suggesting functional and structural changes in human myenteric neurons in specimens of colon from patients receiving anticancer chemotherapy. These changes may contribute to the causation of gastrointestinal symptoms experienced by chemotherapy\u2010treated patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12795","type":"journal-article","created":{"date-parts":[[2016,2,25]],"date-time":"2016-02-25T04:41:28Z","timestamp":1456375288000},"page":"975-984","source":"Crossref","is-referenced-by-count":33,"title":["Electrophysiological and morphological changes in colonic myenteric neurons from chemotherapy\u2010treated patients: a pilot study"],"prefix":"10.1111","volume":"28","author":[{"given":"S. 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