{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T17:50:35Z","timestamp":1789926635295,"version":"4.0.1"},"reference-count":11,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,2,23]],"date-time":"2016-02-23T00:00:00Z","timestamp":1456185600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K23"],"award-info":[{"award-number":["K23"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DK103911"],"award-info":[{"award-number":["DK103911"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30 DK084567"],"award-info":[{"award-number":["P30 DK084567"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 Dk 92179"],"award-info":[{"award-number":["R01 Dk 92179"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Gastrointestinal (<jats:styled-content style=\"fixed-case\">GI<\/jats:styled-content>) and non\u2010<jats:styled-content style=\"fixed-case\">GI<\/jats:styled-content> disorders are associated with altered intestinal permeability, which can be measured <jats:italic>in\u00a0vivo<\/jats:italic> by urinary excretion after oral lactulose and mannitol ingestion. Inadvertent dietary consumption of <jats:sup>12<\/jats:sup>Carbon (<jats:sup>12<\/jats:sup>C, regular) mannitol in food or from other sources may interfere with the test's interpretation. <jats:sup>13<\/jats:sup>Carbon (<jats:sup>13<\/jats:sup>C) constitutes 1% of carbon in nature and <jats:sup>13<\/jats:sup>C mannitol is a stable isotope. Our aim was to determine the performance of <jats:sup>13<\/jats:sup>C mannitol for measurement of intestinal permeability.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ten healthy volunteers underwent intestinal permeability assay using coadministered <jats:sup>12<\/jats:sup>C mannitol, <jats:sup>13<\/jats:sup>C mannitol and lactulose, followed by timed urine collections. Urinary sugar concentrations were measured using tandem high performance liquid chromatography\u2013mass spectrometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key results<\/jats:title><jats:p>We found that <jats:sup>13<\/jats:sup>C mannitol can be distinguishable from <jats:sup>12<\/jats:sup>C mannitol on tandem mass spectrometry. In addition, <jats:sup>13<\/jats:sup>C mannitol had ~20\u2010fold lower baseline contamination compared to <jats:sup>12<\/jats:sup>C mannitol. We describe here the <jats:sup>13<\/jats:sup>C mannitol assay method for the measurement of intestinal permeability.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>In conclusion, <jats:sup>13<\/jats:sup>C mannitol is superior to <jats:sup>12<\/jats:sup>C mannitol for measurement of intestinal permeability. It avoids issues with baseline contamination and erratic excretions during the testing period.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12802","type":"journal-article","created":{"date-parts":[[2016,2,26]],"date-time":"2016-02-26T03:21:24Z","timestamp":1456456884000},"page":"1114-1119","source":"Crossref","is-referenced-by-count":55,"title":["<sup>13<\/sup>C mannitol as a novel biomarker for measurement of intestinal permeability"],"prefix":"10.1111","volume":"28","author":[{"given":"M.","family":"Grover","sequence":"first","affiliation":[{"name":"Division of Gastroenterology and Hepatology Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Camilleri","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Hines","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Burton","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Ryks","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Wadhwa","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Sundt","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Dyer","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. J.","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,2,23]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2012.01921.x"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(95)90708-4"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2012.08.031"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.49.5.650"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2012.01969.x"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/apt.12147"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.114.201673"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(98)70636-5"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1351\/pac199971081593"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00168.2011"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-015-6730-y"}],"container-title":["Neurogastroenterology &amp; Motility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnmo.12802","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nmo.12802","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T11:01:37Z","timestamp":1696503697000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/nmo.12802"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,2,23]]},"references-count":11,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2016,7]]}},"alternative-id":["10.1111\/nmo.12802"],"URL":"https:\/\/doi.org\/10.1111\/nmo.12802","archive":["Portico"],"relation":{},"ISSN":["1350-1925","1365-2982"],"issn-type":[{"value":"1350-1925","type":"print"},{"value":"1365-2982","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,2,23]]}}}