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This has not only been associated with metabolic disorders but recent evidence also indicates that chronic fructose consumption can affect neuronal and cognitive function. In this study we investigated the effects of fructose consumption on serotonergic signaling and neuronal activity in the mouse submucous plexus. Male mice were put on a control or fructose (23% solution) diet for 6 weeks or were assigned to a recovery group that received normal water (2 weeks) after 4 weeks of fructose. At the end of the diet, gene expressions and enteric neuronal activity, after depolarization with high K<jats:sup>+<\/jats:sup> and 5\u2010<jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content>, were measured using Ca<jats:sup>2+<\/jats:sup> imaging and <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>, respectively. Even in the lack of gain weight and the absence of changes in duodenal permeability, the total number of 5\u2010<jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content>\u2010responding neurons and the depolarization and 5\u2010<jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content>\u2010evoked Ca<jats:sup>2+<\/jats:sup> amplitudes were significantly lower after fructose consumption. Expression of synaptobrevin Ca<jats:sub>V<\/jats:sub>2.1 and Ca<jats:sub>V<\/jats:sub>2.2 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> did not differ after fructose intake; however, Ca<jats:sub>V<\/jats:sub>2.1 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels were significantly higher in the recovery animals. <jats:styled-content style=\"fixed-case\">SERT mRNA<\/jats:styled-content> concentration, isolated from submucosal plexus containing mucosal epithelium, was significantly decreased after fructose consumption. Chronic fructose consumption impairs serotonergic signaling in the mouse submucous plexus, prior to weight gain and detectable intestinal permeability problems.<\/jats:p>","DOI":"10.1111\/nmo.12827","type":"journal-article","created":{"date-parts":[[2016,4,21]],"date-time":"2016-04-21T12:48:46Z","timestamp":1461242926000},"page":"1438-1442","source":"Crossref","is-referenced-by-count":11,"title":["Fructose consumption impairs serotonergic signaling in the murine enteric nervous system"],"prefix":"10.1111","volume":"28","author":[{"given":"K.","family":"Lowette","sequence":"first","affiliation":[{"name":"Laboratory for Enteric NeuroScience (LENS) University of Leuven Leuven Belgium"},{"name":"Translational Research Center for Gastrointestinal Disorders (TARGID) University of Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.\u2010S.","family":"Desmet","sequence":"additional","affiliation":[{"name":"Laboratory for Enteric NeuroScience (LENS) University of Leuven Leuven Belgium"},{"name":"Translational Research Center for Gastrointestinal Disorders (TARGID) University of Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.M.","family":"Farr\u00e9","sequence":"additional","affiliation":[{"name":"Translational Research Center for Gastrointestinal Disorders (TARGID) University of Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Tack","sequence":"additional","affiliation":[{"name":"Translational Research Center for Gastrointestinal Disorders (TARGID) University of Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Vanden Berghe","sequence":"additional","affiliation":[{"name":"Laboratory for Enteric NeuroScience (LENS) University of Leuven Leuven Belgium"},{"name":"Translational Research Center for Gastrointestinal Disorders (TARGID) University of Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,4,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2014.00088"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2010.12.003"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.23.4.456"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.90245.2008"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2015.00005"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nlm.2009.05.007"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.regpep.2010.11.002"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2005.03155.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00088.2009"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2012.231134"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-1838(00)00127-2"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbrainres.2004.02.007"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1990.tb13017.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2008.01157.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2000.278.2.G273"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12385"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2010-0377"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2013.105"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mcg.0000156403.37240.30"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2005.00673.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1113\/EP085427"}],"container-title":["Neurogastroenterology &amp; 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