{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:15:54Z","timestamp":1791234954577,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2016,4,5]],"date-time":"2016-04-05T00:00:00Z","timestamp":1459814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100004388","name":"Cephalon","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004388","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2016,9]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>Irritable bowel syndrome (IBS<\/jats:styled-content>) physiopathology is multifactorial and roles for both microbiota and bile acid (BA<\/jats:styled-content>) modifications have been proposed. We investigated role of dysbiosis, transit pattern and BA<\/jats:styled-content> metabolism in IBS<\/jats:styled-content>.<\/jats:p><\/jats:sec>Methods<\/jats:title>Clinical data, serum, and stool samples were collected in 15 healthy subjects (HS<\/jats:styled-content>), 16 diarrhea\u2010predominant (IBS<\/jats:styled-content>\u2010D) and 15 constipation\u2010predominant IBS<\/jats:styled-content> (IBS<\/jats:styled-content>\u2010C). Fecal microbiota composition was analyzed by real\u2010time PCR<\/jats:styled-content>. Sera and fecal BA<\/jats:styled-content> profiles, 7\u03b1<\/jats:italic>\u2010C4 levels, and in vitro <\/jats:italic>BA<\/jats:styled-content> transformation activity by fecal microbiota were measured by mass spectrometry. Serum Fibroblast Growth Factor 19 (FGF19) was assayed by ELISA<\/jats:styled-content>.<\/jats:p><\/jats:sec>Keys Results<\/jats:title>Dysbiosis was present in IBS<\/jats:styled-content> patients with an increase in Escherichia coli<\/jats:italic> in IBS<\/jats:styled-content>\u2010D patients (p<\/jats:italic> = 0.03), and an increase in Bacteroides<\/jats:italic> (p<\/jats:italic> = 0.01) and Bifidobacterium<\/jats:italic> (p<\/jats:italic> = 0.04) in IBS<\/jats:styled-content>\u2010C patients. Sera primary and amino\u2010conjugated BA<\/jats:styled-content> were increased in IBS<\/jats:styled-content>\u2010D (63.5 \u00b1 5.5%, p<\/jats:italic> = 0.01 and 78.9 \u00b1 6.3%, p<\/jats:italic> = 0.03) and IBS<\/jats:styled-content>\u2010C patients (55.9 \u00b1 5.5%, p<\/jats:italic> = 0.04 and 65.3 \u00b1 6.5%, p<\/jats:italic> = 0.005) compared to HS<\/jats:styled-content> (37.0 \u00b1 5.8% and 56.7 \u00b1 8.1%). Serum 7\u03b1<\/jats:italic>\u2010C4 and FGF<\/jats:styled-content>19 levels were not different among all three groups. Fecal primary BA<\/jats:styled-content> were increased in IBS<\/jats:styled-content>\u2010D patients compared to HS<\/jats:styled-content>, including chenodeoxycholic acid which has laxative properties (25.6 \u00b1 8.5% vs<\/jats:italic> 3.5 \u00b1 0.6%, p<\/jats:italic> = 0.005). Bile acid deconjugation activity was decreased in IBS<\/jats:styled-content>\u2010D (p<\/jats:italic> = 0.0001) and IBS<\/jats:styled-content>\u2010C (p<\/jats:italic> = 0.003) feces. Abdominal pain was positively correlated with serum (R<\/jats:italic> = 0.635, p<\/jats:italic> < 0.001) and fecal (R<\/jats:italic> = 0.391, p<\/jats:italic> = 0.024) primary BA<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusions & Inferences<\/jats:title>Different sera and fecal BA<\/jats:styled-content> profiles in IBS<\/jats:styled-content> patients could be secondary to dysbiosis and further differences between IBS<\/jats:styled-content>\u2010C and IBS<\/jats:styled-content>\u2010D could explain stool patterns. This study opens new fields in IBS<\/jats:styled-content> physiopathology and suggests that modification of BA<\/jats:styled-content> profiles could have therapeutic potential.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12829","type":"journal-article","created":{"date-parts":[[2016,4,10]],"date-time":"2016-04-10T12:57:13Z","timestamp":1460293033000},"page":"1330-1340","source":"Crossref","is-referenced-by-count":120,"title":["Interplay between bile acid metabolism and microbiota in irritable bowel syndrome"],"prefix":"10.1111","volume":"28","author":[{"given":"M.","family":"Dior","sequence":"first","affiliation":[{"name":"ERL U1157\/UMR 7203. Inserm Paris VI University Paris France"},{"name":"Department of Gastroenterology Louis Mourier Hospital AP\u2010HP Colombes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Delagr\u00e8verie","sequence":"additional","affiliation":[{"name":"ERL U1157\/UMR 7203. Inserm Paris VI University Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Duboc","sequence":"additional","affiliation":[{"name":"ERL U1157\/UMR 7203. Inserm Paris VI University Paris France"},{"name":"Department of Gastroenterology Louis Mourier Hospital AP\u2010HP Colombes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Jouet","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Louis Mourier Hospital AP\u2010HP Colombes France"},{"name":"Inserm U987 Physiopathologie et pharmacologie Clinique de la douleur H\u00f4pital Ambroise Par\u00e9 AP\u2010HP Boulogne France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Coffin","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Louis Mourier Hospital AP\u2010HP Colombes France"},{"name":"Inserm U987 Physiopathologie et pharmacologie Clinique de la douleur H\u00f4pital Ambroise Par\u00e9 AP\u2010HP Boulogne France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Brot","sequence":"additional","affiliation":[{"name":"ERL U1157\/UMR 7203. Inserm Paris VI University Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Humbert","sequence":"additional","affiliation":[{"name":"ERL U1157\/UMR 7203. Inserm Paris VI University Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Trugnan","sequence":"additional","affiliation":[{"name":"ERL U1157\/UMR 7203. Inserm Paris VI University Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Seksik","sequence":"additional","affiliation":[{"name":"ERL U1157\/UMR 7203. Inserm Paris VI University Paris France"},{"name":"Department of Gastroenterology and Nutrition Saint Antoine Hospital AP\u2010HP Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Sokol","sequence":"additional","affiliation":[{"name":"ERL U1157\/UMR 7203. Inserm Paris VI University Paris France"},{"name":"Department of Gastroenterology and Nutrition Saint Antoine Hospital AP\u2010HP Paris France"},{"name":"INRA UMR1319 Micalis Jouy\u2010en\u2010Josas France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Rainteau","sequence":"additional","affiliation":[{"name":"ERL U1157\/UMR 7203. Inserm Paris VI University Paris France"},{"name":"Biochemistry Department Saint Antoine Hospital AP\u2010HP Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.\u2010M.","family":"Sabate","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Louis Mourier Hospital AP\u2010HP Colombes France"},{"name":"Inserm U987 Physiopathologie et pharmacologie Clinique de la douleur H\u00f4pital Ambroise Par\u00e9 AP\u2010HP Boulogne France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,4,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gtc.2010.12.006"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gcb.2009.07.006"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00537.2011"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2006.10.026"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2006.00875.x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCG.0000000000000418"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2007.04.005"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-230X-9-95"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2005.40312.x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.07.043"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.5009\/gnl14344"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2013-305965"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2013.04.020"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2009.04081.x"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365521003702734"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens3010014"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0114379"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.R500013-JLR200"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2012.01893.x"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.11.061"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0804812105"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.20903"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2009.00671.x"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2009.01288.x"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2012.05.015"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2012-302578"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.90343.2008"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2009.04.024"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2009.10.020"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.49.6.835"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2011.01803.x"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2011-301501"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.32.9.1004"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2012.05.006"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1586\/egh.09.49"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2014.215"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/apt.13065"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365527809181914"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2010.07.052"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0b013e31816920a6"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-011-3798-x"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00178.2014"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12010-012-9904-4"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00194.2001"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.30.3.383"},{"issue":"8","key":"e_1_2_10_47_1","first-page":"39","article-title":"Is the irritable bowel really irritable?","volume":"23","author":"Coremans G","year":"1991","journal-title":"Ital J Gastroenterol"}],"container-title":["Neurogastroenterology & Motility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnmo.12829","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nmo.12829","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T14:04:48Z","timestamp":1696169088000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/nmo.12829"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4,5]]},"references-count":46,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2016,9]]}},"alternative-id":["10.1111\/nmo.12829"],"URL":"https:\/\/doi.org\/10.1111\/nmo.12829","archive":["Portico"],"relation":{},"ISSN":["1350-1925","1365-2982"],"issn-type":[{"value":"1350-1925","type":"print"},{"value":"1365-2982","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,4,5]]}}}