{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T06:15:02Z","timestamp":1695622502644},"reference-count":17,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,4,2]],"date-time":"2016-04-02T00:00:00Z","timestamp":1459555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Histological assessment of the interstitial cells of Cajal (<jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content>s) in the bowel is important for diagnosing patients with gastrointestinal neuromuscular diseases (<jats:styled-content style=\"fixed-case\">GINMD<\/jats:styled-content>). Although the International Working Group on <jats:styled-content style=\"fixed-case\">GINMD<\/jats:styled-content> proposed reporting a decrease in <jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content> number of more than 50%, quantitative methods used in literature are not practical for daily routine of the pathologist. Consequently, this study presents a straightforward semiquantitative estimation method for myenteric <jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content>s of the bowel.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Formalin\u2010fixed paraffin\u2010embedded sections from small bowel (<jats:italic>n<\/jats:italic> = 87) and colon (<jats:italic>n<\/jats:italic> = 159) were collected to create two control groups and four groups composed of patients with gastrointestinal motility disorders. The control groups included material of resection and autopsy origin, respectively. Samples were stained with <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>117 (c\u2010kit) antibody to estimate the myenteric <jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content> network. Scores of two observers were compared to analyze inter\u2010 and intraobserver agreement and reliability.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Interobserver reliability was almost perfect for small bowel (intraclass correlation coefficient 0.847; 95% confidence interval [<jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>]: 0.774\u20130.897) and substantial for colon (0.683; 95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>: 0.591\u20130.758). Almost perfect intraobserver reliability was found (intraclass correlation coefficient 0.918; 95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>: 0.874\u20130.947). The small bowel showed more myenteric <jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content>s than the colon. Neither significant differences between colonic regions were found nor were there any differences in the orientation of the sections.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>The proposed estimation method for the myenteric <jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content> network showed generally good agreement and reliability. As the method is semiquantitative, simple, and capable to differentiate between normal and diseased tissue, it can be used in routine diagnostics of gastrointestinal neuromuscular disorders.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12831","type":"journal-article","created":{"date-parts":[[2016,4,2]],"date-time":"2016-04-02T21:59:59Z","timestamp":1459634399000},"page":"1261-1267","source":"Crossref","is-referenced-by-count":7,"title":["Practical and reproducible estimation of myenteric interstitial cells of Cajal in the bowel for diagnostic purposes"],"prefix":"10.1111","volume":"28","author":[{"given":"M.","family":"den Braber\u2010Ymker","sequence":"first","affiliation":[{"name":"Department of Pathology Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Heijker","sequence":"additional","affiliation":[{"name":"Department of Pathology Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Lammens","sequence":"additional","affiliation":[{"name":"Department of Pathology Radboud University Medical Center Nijmegen The Netherlands"},{"name":"Department of Pathology Antwerp University Hospital University of Antwerp Edegem Belgium"},{"name":"MIPRO University of Antwerp Antwerp Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. 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