{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:41:47Z","timestamp":1789432907544,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,7,5]],"date-time":"2016-07-05T00:00:00Z","timestamp":1467676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81070308\/H0308"],"award-info":[{"award-number":["81070308\/H0308"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Nesfatin\u20101, a recently identified satiety molecule derived from nucleobindin 2 (<jats:styled-content style=\"fixed-case\">NUCB<\/jats:styled-content>2), is associated with visceral hypersensitivity in rats and is expressed in the amygdala. We tested the hypothesis that nesfatin\u20101 expression in the amygdala is involved in the pathogenesis of irritable bowel syndrome (<jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content>) visceral hypersensitivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>An animal model of <jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content>\u2010like visceral hypersensitivity was established using maternal separation (<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>) during postnatal days 2\u201316. The role of nesfatin\u20101 in the amygdala on visceral sensitivity was evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Rats subjected to <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> showed a significantly increased mean abdominal withdrawal reflex (<jats:styled-content style=\"fixed-case\">AWR<\/jats:styled-content>) score and electromyographic (<jats:styled-content style=\"fixed-case\">EMG<\/jats:styled-content>) activity at 40, 60, and 80 mmHg colorectal distension. Plasma concentrations of nesfatin\u20101 and corticosterone were significantly higher than in non\u2010handled (<jats:styled-content style=\"fixed-case\">NH<\/jats:styled-content>) rats. <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> and protein expression of nesfatin\u20101\/<jats:styled-content style=\"fixed-case\">NUCB<\/jats:styled-content>2 in the amygdala were increased in <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> rats, but not in <jats:styled-content style=\"fixed-case\">NH<\/jats:styled-content> rats. In <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> rats, <jats:styled-content style=\"fixed-case\">AWR<\/jats:styled-content> scores and <jats:styled-content style=\"fixed-case\">EMG<\/jats:styled-content> activity were significantly decreased after anti\u2010nesfatin\u20101\/<jats:styled-content style=\"fixed-case\">NUCB<\/jats:styled-content>2 injection. In normal rats, mean <jats:styled-content style=\"fixed-case\">AWR<\/jats:styled-content> score, <jats:styled-content style=\"fixed-case\">EMG<\/jats:styled-content> activity, and corticosterone expression were significantly increased after nesfatin\u20101 injection into the amygdala. Nesfatin\u20101\u2010induced visceral hypersensitivity was abolished following application of glucocorticoid receptor (<jats:styled-content style=\"fixed-case\">GR<\/jats:styled-content>) and mineralocorticoid receptor (<jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content>) antagonists.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>Elevated expression of nesfatin\u20101\/<jats:styled-content style=\"fixed-case\">NUCB<\/jats:styled-content>2 in the amygdala in <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> rats suggests a potential role in the pathogenesis of visceral hypersensitivity, which could potentially take place via activation of <jats:styled-content style=\"fixed-case\">GR<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">MR<\/jats:styled-content> signaling pathways.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12853","type":"journal-article","created":{"date-parts":[[2016,7,6]],"date-time":"2016-07-06T04:19:23Z","timestamp":1467778763000},"page":"1545-1553","source":"Crossref","is-referenced-by-count":15,"title":["Nesfatin\u20101\/<scp>NUCB<\/scp>2 in the amygdala influences visceral sensitivity via glucocorticoid and mineralocorticoid receptors in male maternal separation rats"],"prefix":"10.1111","volume":"28","author":[{"given":"X.\u2010P.","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Gastroenterology First Affiliated Hospital of Nanjing Medical University Nanjing China"},{"name":"Third People's Hospital of Zigong Zigong Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Sha","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology First Affiliated Hospital of Nanjing Medical University Nanjing 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