{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T01:48:00Z","timestamp":1781920080313,"version":"3.54.5"},"reference-count":29,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,6,14]],"date-time":"2016-06-14T00:00:00Z","timestamp":1465862400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21300005131405"],"award-info":[{"award-number":["21300005131405"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2014M560565"],"award-info":[{"award-number":["2014M560565"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Oxytocin (<jats:styled-content style=\"fixed-case\">OT<\/jats:styled-content>) reduces rat duodenal tone and mouse intestinal transit; however, the underlying mechanisms are not totally understood. Consequently, this study was designed to investigate the influence of <jats:styled-content style=\"fixed-case\">OT<\/jats:styled-content> on spontaneous mechanical activity and neurally evoked responses, to characterize the mechanisms of the action, and to determine the distribution of the <jats:styled-content style=\"fixed-case\">OT<\/jats:styled-content> receptor (<jats:styled-content style=\"fixed-case\">OTR<\/jats:styled-content>) in rat proximal colonic muscle strips.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The organ bath technique with electrical field stimulation, western blotting, and immunofluorescence were used.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>In rat proximal colon, exogenous <jats:styled-content style=\"fixed-case\">OT<\/jats:styled-content> induced a concentration\u2010dependent reduction of the spontaneous mechanical activity without affecting the resting basal tone, which was abolished by atosiban, an <jats:styled-content style=\"fixed-case\">OTR<\/jats:styled-content> antagonist, by tetrodotoxin (<jats:styled-content style=\"fixed-case\">TTX<\/jats:styled-content>), a neural blocker or by N<jats:italic>\u03c9<\/jats:italic>\u2010propyl\u2010<jats:sc>l<\/jats:sc>\u2010arginine hydrochloride, an inhibitor of neuronal nitric oxide synthase (<jats:styled-content style=\"fixed-case\">nNOS<\/jats:styled-content>). The inhibitory effects of <jats:styled-content style=\"fixed-case\">OT<\/jats:styled-content> were not affected by atropine or the vasoactive intestinal peptide (<jats:styled-content style=\"fixed-case\">VIP<\/jats:styled-content>) receptor antagonist [Lys1, Pro2,5, Arg3,4, Tyr6]\u2010VIP (<jats:styled-content style=\"fixed-case\">VIPH<\/jats:styled-content>yb). Proximal colon responses to electrical field stimulation were characterized by nonadrenergic, noncholinergic (<jats:styled-content style=\"fixed-case\">NANC<\/jats:styled-content>) relaxation, which was followed by an off\u2010contraction. Oxytocin enhanced only <jats:styled-content style=\"fixed-case\">NANC<\/jats:styled-content> relaxation. Oxytocin stimulated spontaneous <jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content> release from the longitudinal muscle myenteric plexus preparation of rat proximal colon. Western blot and immunohistochemistry experiments showing the presence of the <jats:styled-content style=\"fixed-case\">OTR<\/jats:styled-content> in proximal colon, and its co\u2010localization with <jats:styled-content style=\"fixed-case\">nNOS<\/jats:styled-content> established that myenteric nitrergic neurons express OTR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>The activation of <jats:styled-content style=\"fixed-case\">OTR<\/jats:styled-content> located on nitrergic neurons may negatively modulate colonic spontaneous contraction and enhance electrically evoked <jats:styled-content style=\"fixed-case\">NANC<\/jats:styled-content> relaxation through excitation of <jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content> release.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12883","type":"journal-article","created":{"date-parts":[[2016,6,15]],"date-time":"2016-06-15T12:44:55Z","timestamp":1465994695000},"page":"1815-1823","source":"Crossref","is-referenced-by-count":15,"title":["Role of the nitrergic pathway in motor effects of oxytocin in rat proximal colon"],"prefix":"10.1111","volume":"28","author":[{"given":"R.","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Physiology Shandong University School of Medicine Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. Y.","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Physiology Shandong University School of Medicine Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Y.","family":"Suo","sequence":"additional","affiliation":[{"name":"Department of Physiology Shandong University School of Medicine Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Physiology Shandong University School of Medicine Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Y.","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Physiology Shandong University School of Medicine Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X. Y.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Genetics Shandong University School of Medicine Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Y.","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Physiology Shandong University School of Medicine Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21872"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.regpep.2006.03.008"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.regpep.2003.12.017"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2012.07702.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v9.i1.165"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00176.2014"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12469"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4165"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-010-0880-7"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2009.01282.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00328.2009"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2012.06.007"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2004.10.005"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1530\/eje.0.1360531"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-003-0690-y"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-230X-6-11"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4892(02)00225-4"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(78)90070-5"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1988.tb26993.x"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1994.tb13129.x"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(90)91605-R"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1993.tb12808.x"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0707609"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.23243"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M314337200"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2010.177"},{"key":"e_1_2_9_28_1","first-page":"293","article-title":"Neuronal nitric oxide synthase and nerve growth factor expression in the enteric nervous system","volume":"51","author":"Thippeswamy T","year":"2005","journal-title":"Cell Mol Biol"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12330"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/scrt387"}],"container-title":["Neurogastroenterology &amp; Motility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnmo.12883","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnmo.12883","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nmo.12883","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T17:05:06Z","timestamp":1696266306000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/nmo.12883"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6,14]]},"references-count":29,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["10.1111\/nmo.12883"],"URL":"https:\/\/doi.org\/10.1111\/nmo.12883","archive":["Portico"],"relation":{},"ISSN":["1350-1925","1365-2982"],"issn-type":[{"value":"1350-1925","type":"print"},{"value":"1365-2982","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6,14]]}}}