{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T20:15:55Z","timestamp":1789848955413,"version":"4.0.1"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,9,12]],"date-time":"2016-09-12T00:00:00Z","timestamp":1473638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004271","name":"Sapienza Universit\u00e0 di Roma","doi-asserted-by":"publisher","award":["000324_13 Severi Sapienza"],"award-info":[{"award-number":["000324_13 Severi Sapienza"]}],"id":[{"id":"10.13039\/501100004271","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Changes in intestinal motility are likely to contribute to irritable bowel syndrome (<jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content>) pathophysiology. The aim of the study was to investigate the effects of <jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content> mucosal supernatants on human colonic muscle contractility.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Supernatants were obtained from biopsies of 18 <jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content> patients\u2014nine with constipation (<jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content>\u2010C) and nine with diarrhea\u2010predominant <jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content>\u2010D)\u2014and nine asymptomatic subjects, used as controls. Colonic circular smooth muscle strips or isolated cells (<jats:styled-content style=\"fixed-case\">SMC<\/jats:styled-content>) were exposed to control or <jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content> supernatants. Spontaneous phasic contractions on strips and morphofunctional parameters on cells were evaluated in basal conditions and in response to acetylcholine (Ach). Incubation with <jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content> supernatants was also conducted in the presence of antagonists and inhibitors (namely histamine, protease and prostaglandin antagonists, nuclear factor\u2010kappa B inhibitor, catalase, <jats:styled-content style=\"fixed-case\">NADPH<\/jats:styled-content> oxidase inhibitor, and the <jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>\u2010 and\/or <jats:styled-content style=\"fixed-case\">cGMP<\/jats:styled-content>\u2010cyclase inhibitors).<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Exposure to <jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content>\u2010C and <jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content>\u2010D supernatants induced a significant reduction in basal tone and Ach\u2010elicited contraction of muscle strips and a significant shortening and impairment of Ach contraction of <jats:styled-content style=\"fixed-case\">SMC<\/jats:styled-content>s. The <jats:styled-content style=\"fixed-case\">NADPH<\/jats:styled-content> oxidase inhibitor prevented the effect of supernatants, while the protease antagonist only <jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content>\u2010C effect. No effect was observed with the other antagonists and inhibitors. Dilution of <jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content>\u2010D supernatants partially restored the effects only on <jats:styled-content style=\"fixed-case\">SMC<\/jats:styled-content>s, whereas dilution of <jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content>\u2010C supernatants significantly reverted the effects on muscle strips and Ach\u2010elicited response on <jats:styled-content style=\"fixed-case\">SMC<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>Supernatants from mucosal biopsies of <jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content> patients reduce colonic contractility. The observed impairment was concentration dependent, likely occurring through intracellular oxidative stress damage, involving different neuromotor mechanisms depending on the <jats:styled-content style=\"fixed-case\">IBS<\/jats:styled-content> subtype.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12928","type":"journal-article","created":{"date-parts":[[2016,9,13]],"date-time":"2016-09-13T00:43:01Z","timestamp":1473727381000},"source":"Crossref","is-referenced-by-count":15,"title":["Supernatants of irritable bowel syndrome mucosal biopsies impair human colonic smooth muscle contractility"],"prefix":"10.1111","volume":"29","author":[{"given":"M. P.","family":"Guarino","sequence":"first","affiliation":[{"name":"Gastroenterology Department University Campus Bio\u2010medico Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Barbara","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences University of Bologna Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Cicenia","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Medical Specialties University Sapienza Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Altomare","sequence":"additional","affiliation":[{"name":"Gastroenterology Department University Campus Bio\u2010medico Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. R.","family":"Barbaro","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences University of Bologna Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Cocca","sequence":"additional","affiliation":[{"name":"Gastroenterology Department University Campus Bio\u2010medico Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Scirocco","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Medical Specialties University Sapienza Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7777-2936","authenticated-orcid":false,"given":"C.","family":"Cremon","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences University of Bologna Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Emerenziani","sequence":"additional","affiliation":[{"name":"Gastroenterology Department University Campus Bio\u2010medico Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Stanghellini","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences University of Bologna Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Cicala","sequence":"additional","affiliation":[{"name":"Gastroenterology Department University Campus Bio\u2010medico Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Severi","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Medical Specialties University Sapienza Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,9,12]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"71","article-title":"The epidemiology of irritable bowel syndrome","volume":"6","author":"Canavan C","year":"2014","journal-title":"J Clin Epidemiol"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2006.03.008"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2013.410"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12670"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2007.140806"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.47.6.804"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2004.02284.x"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2006.00672.x"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2010.440"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2006.11.039"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2009.07.005"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12011"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2015.04.011"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.3780030108"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1540\/jsmr.43.43"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2004.04.006"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.03.049"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12316"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI29255"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2010.4"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12000"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2013.06.001"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22053"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2012.09.022"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2015.0954"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2003.11.055"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00385.2013"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2004.06.016"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2012.01927.x"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.0793e.x"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.11.033"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2008.94"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2009.00394.x"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.25105"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2014.285148"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2001.280.4.G546"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00462.2003"}],"container-title":["Neurogastroenterology &amp; Motility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnmo.12928","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnmo.12928","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nmo.12928","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T21:50:11Z","timestamp":1695505811000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/nmo.12928"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9,12]]},"references-count":37,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,2]]}},"alternative-id":["10.1111\/nmo.12928"],"URL":"https:\/\/doi.org\/10.1111\/nmo.12928","archive":["Portico"],"relation":{},"ISSN":["1350-1925","1365-2982"],"issn-type":[{"value":"1350-1925","type":"print"},{"value":"1365-2982","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9,12]]},"article-number":"e12928"}}