{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T17:34:23Z","timestamp":1758476063242,"version":"3.41.2"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,9,12]],"date-time":"2016-09-12T00:00:00Z","timestamp":1473638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Institutional Intramural Research Committee"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To derive objective values for the diagnosis of Hirschsprung disease (<jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>) from a comparison of the morphometric profile of large intestinal neuronal plexuses in normal perinatal autopsies and surgical specimens of <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A cross\u2010sectional comparative study with 40 subjects each in (i) non\u2010<jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> perinatal group encompassing neonates and stillborn babies beyond 30\u00a0weeks of gestation on whom autopsies were conducted and (ii) <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> group comprising all patients clinicoradiologically diagnosed as <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>. The morphometric assessment was done on hematoxylin\u2010and\u2010eosin\u2010stained sections.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>The morphometric profile in terms of average number of ganglia\/linear mm of colon, interganglion distance, number of ganglion cells\/ganglion, average ganglion cell length, ganglion cell nuclear area, ganglion cell nuclear diameter, nerve trunk thickness, and density has been outlined. On comparison with the neuroanatomically normal zone of <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>, the cut\u2010offs to identify hypertrophic nerve trunks (nerve trunk thickness of &gt;37.85\u00a0\u03bcm) and reduced number of ganglia (number of ganglia\/linear mm of colon &lt;2.05 and interganglion distance of &gt;229\u00a0\u03bcm) were derived.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>The determined objective values, after testing on diagnostic rectal biopsies, may serve to formulate a diagnostic algorithm along with immunostaining for diagnosis of <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> in colorectal specimens.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.12939","type":"journal-article","created":{"date-parts":[[2016,9,13]],"date-time":"2016-09-13T02:26:46Z","timestamp":1473733606000},"source":"Crossref","is-referenced-by-count":7,"title":["Morphometric profile of large intestinal neuronal plexuses in normal perinatal autopsies and Hirschsprung disease"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1234-0451","authenticated-orcid":false,"given":"H.","family":"Subramanian","sequence":"first","affiliation":[{"name":"Department of Pathology (Histopathology section) Jawaharlal Institute of Postgraduate Medical Education and Research Pondicherry India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. A.","family":"Badhe","sequence":"additional","affiliation":[{"name":"Department of Pathology (Histopathology section) Jawaharlal Institute of Postgraduate Medical Education and Research Pondicherry India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. C.","family":"Toi","sequence":"additional","affiliation":[{"name":"Department of Pathology (Histopathology section) Jawaharlal Institute of Postgraduate Medical Education and Research Pondicherry India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Sambandan","sequence":"additional","affiliation":[{"name":"Department of Paediatric Surgery Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER) Pondicherry India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,9,12]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2012.32"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.sempedsurg.2009.07.002"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00383-013-3351-3"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.sempedsurg.2012.07.005"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00383-010-2848-2"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-1318.2009.02031.x"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2003.08.028"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2007.01.035"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s100240010115"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.2350\/12-12-1282-OA.1"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.4103\/0377-4929.81589"},{"key":"e_1_2_12_13_1","first-page":"156","article-title":"Prenatal development of the myenteric plexus in human sigmoid colon","volume":"30","author":"Singh S","year":"2013","journal-title":"J Morphol Sci"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2009.12.012"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1309\/T7REY1N43FML7AA8"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.sempedsurg.2012.07.003"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2006.08.004"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.4132\/KoreanJPathol.2010.44.2.162"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-010-1522-9"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12303"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mcg.0000173856.84814.37"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.2350\/06-06-0109.1"},{"key":"e_1_2_12_23_1","first-page":"721","article-title":"Increased submucosal nerve trunk caliber in aganglionosis: a \u201cpositive\u201d and objective finding in suction biopsies and segmental resections in Hirschsprung's disease","volume":"122","author":"Monforte\u2010Mu\u00f1oz H","year":"1998","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_12_24_1","first-page":"195","article-title":"Morphometry of nerve trunks in Hirschsprung disease","volume":"8","author":"Bandyopadhyay R","year":"2003","journal-title":"J Indian Assoc Pediatr Surg"}],"container-title":["Neurogastroenterology &amp; Motility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnmo.12939","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnmo.12939","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nmo.12939","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T07:29:15Z","timestamp":1696490955000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/nmo.12939"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9,12]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1111\/nmo.12939"],"URL":"https:\/\/doi.org\/10.1111\/nmo.12939","archive":["Portico"],"relation":{},"ISSN":["1350-1925","1365-2982"],"issn-type":[{"type":"print","value":"1350-1925"},{"type":"electronic","value":"1365-2982"}],"subject":[],"published":{"date-parts":[[2016,9,12]]},"article-number":"e12939"}}