{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:06:38Z","timestamp":1790625998505,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,3,3]],"date-time":"2017-03-03T00:00:00Z","timestamp":1488499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002426","name":"Fondazione Telethon","doi-asserted-by":"publisher","award":["GGP15171"],"award-info":[{"award-number":["GGP15171"]}],"id":[{"id":"10.13039\/501100002426","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004740","name":"Fondazione del Monte di Bologna e Ravenna","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004740","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Serotonin plays a pivotal role in regulating gut motility, visceral sensitivity, and fluid secretion via specific receptors. Among these receptors, 5\u2010<jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content><jats:sub>4<\/jats:sub>exerts a prominent control on gut motor function. Although the prokinetic effect exerted by 5\u2010<jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content><jats:sub>4<\/jats:sub>agonists is well known, the cellular sites of 5\u2010<jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content><jats:sub>4<\/jats:sub>expression remain poorly understood in large mammals, e.g., horses. In this study, we evaluated the distribution of 5\u2010<jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content><jats:sub>4<\/jats:sub>in the horse intestine and in foals with enteric aganglionosis, reminiscent of human Hirschsprung's disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The intestine and spinal ganglia were obtained from three healthy horses and two foals with hereditary ileocolonic aganglionosis. Tissues were processed for immunohistochemistry using a specific antibody to 5\u2010<jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content><jats:sub>4<\/jats:sub>and a variety of neuronal markers. Myenteric and submucosal plexus 5\u2010<jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content><jats:sub>4<\/jats:sub>\u2010immunoreactive (<jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>) neurons were quantified as relative percentage (mean\u00b1<jats:styled-content style=\"fixed-case\">SD<\/jats:styled-content>) to the total number of neurons counted. Furthermore, the density of 5\u2010<jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content><jats:sub>4<\/jats:sub>\u2010<jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>nerve fibers was evaluated in the mucosa and<jats:italic>tunica muscularis<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>The 5\u2010<jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content><jats:sub>4<\/jats:sub>immunoreactivity was localized to large percentages of myenteric neurons ranging from 28\u00b19% (descending colon) to 63\u00b119% (ileum), and submucosal neurons ranging from 54\u00b16% (ileum) to 68\u00b114% (duodenum). The 5\u2010<jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content><jats:sub>4<\/jats:sub>\u2010immunoreactivity was co\u2010expressed by some substance P\u2010<jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>(<jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>) spinal ganglion neurons and extrinsic sensory fibers of aganglionic foals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>The presence of 5\u2010<jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content><jats:sub>4<\/jats:sub>in many enteric and extrinsic sensory neurons and nerve fibers provides solid morphological evidence of the cellular sites of 5\u2010<jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content><jats:sub>4<\/jats:sub>expression in horses. The evidence of<jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>sensory neurons positive for 5\u2010<jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content><jats:sub>4<\/jats:sub>suggests its role in visceral sensitivity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.13045","type":"journal-article","created":{"date-parts":[[2017,3,3]],"date-time":"2017-03-03T05:46:50Z","timestamp":1488520010000},"source":"Crossref","is-referenced-by-count":14,"title":["Localization of the 5\u2010hydroxytryptamine 4 receptor in equine enteric neurons and extrinsic sensory fibers"],"prefix":"10.1111","volume":"29","author":[{"given":"F.","family":"Giancola","sequence":"first","affiliation":[{"name":"Department of Veterinary Medical Sciences (UNI EN ISO 9001:2008) University of Bologna Bologna Italy"},{"name":"Department of Medical and Surgical Sciences University of Bologna Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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