{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T22:37:18Z","timestamp":1791499038780,"version":"4.3.4"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,3,23]],"date-time":"2017-03-23T00:00:00Z","timestamp":1490227200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In the gastrointestinal tract of several species, facilitating 5\u2010<jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content><jats:sub>4<\/jats:sub> receptors were proposed on myenteric cholinergic neurons innervating smooth muscle by <jats:italic>in\u00a0vitro<\/jats:italic> study of the effect of the selective 5\u2010<jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content><jats:sub>4<\/jats:sub> receptor agonist prucalopride on submaximal cholinergic contractions. This was not yet established in the murine gastrointestinal tract.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In circular smooth muscle strips from murine fundus, jejunum and colon, contractions were induced by electrical field stimulation in the presence of guanethidine, L\u2010<jats:styled-content style=\"fixed-case\">NAME<\/jats:styled-content> and for colon also <jats:styled-content style=\"fixed-case\">MRS<\/jats:styled-content> 2500. Submaximal contractions were induced to study the influence of prucalopride.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Electrical field stimulation at reduced voltage induced reproducible submaximal neurogenic and cholinergic contractions as the contractions were abolished by tetrodotoxin and atropine. Hexamethonium had no systematic inhibitory effect but mecamylamine reduced the responses, suggesting that part of the cholinergic response is due to activation of preganglionic neurons. Prucalopride concentration\u2010dependently increased the submaximal cholinergic contractions in the three tissue types, reaching maximum from 0.03\u00a0\u03bcmol\/L onwards. The facilitation in the different series with 0.03\u00a0\u03bcmol\/L prucalopride ranged from 41% to 104%, 30% to 76% and 24% to 74% in fundus, jejunum, and colon, respectively. The effect of 0.03\u00a0\u03bcmol\/L prucalopride was concentration\u2010dependently inhibited by <jats:styled-content style=\"fixed-case\">GR<\/jats:styled-content> 113808.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Inferences<\/jats:title><jats:p>In the murine gastrointestinal tract, activation of 5\u2010<jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content><jats:sub>4<\/jats:sub> receptors with prucalopride enhances cholinergic contractions, illustrating facilitation of myenteric cholinergic neurotransmission. The degree of enhancement with prucalopride is of similar magnitude as previously reported in other species, but the effective concentrations are lower than those needed in the gastrointestinal tract of other species.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.13064","type":"journal-article","created":{"date-parts":[[2017,3,24]],"date-time":"2017-03-24T06:03:31Z","timestamp":1490335411000},"source":"Crossref","is-referenced-by-count":11,"title":["5\u2010<scp>HT<\/scp><sub>4<\/sub> receptors facilitate cholinergic neurotransmission throughout the murine gastrointestinal tract"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6329-1103","authenticated-orcid":false,"given":"V.","family":"Pauwelyn","sequence":"first","affiliation":[{"name":"Department of Pharmacology \u2010 Heymans Institute Ghent University  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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