{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T01:28:42Z","timestamp":1790731722854,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2017,5,19]],"date-time":"2017-05-19T00:00:00Z","timestamp":1495152000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Experimental and clinical studies have shown that myenteric neuron cell death during infection with <jats:italic>Trypanosoma cruzi<\/jats:italic> mainly occurs in the esophagus and colon, resulting in megaesophagus and megacolon, respectively<jats:bold>.<\/jats:bold> Evidence suggests that the cyclooxygenase enzyme (<jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>) is involved in the <jats:italic>T.\u00a0cruzi<\/jats:italic> invasion process. The use of low\u2010dose aspirin (<jats:styled-content style=\"fixed-case\">ASA<\/jats:styled-content>), a <jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>\u20101\/<jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>\u20102 inhibitor, has been shown to reduce infection with <jats:italic>T.\u00a0cruzi<\/jats:italic>. Therefore, in this study, we evaluated the effects of treatment with low\u2010dose <jats:styled-content style=\"fixed-case\">ASA<\/jats:styled-content> on myenteric colonic neurons during murine infection with <jats:italic>T.\u00a0cruzi<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Swiss mice were assigned into groups treated with either phosphate\u2010buffered saline or low doses of <jats:styled-content style=\"fixed-case\">ASA<\/jats:styled-content> during the acute phase (20\u00a0mg\/kg <jats:styled-content style=\"fixed-case\">ASA<\/jats:styled-content>) and chronic phase (50\u00a0mg\/kg <jats:styled-content style=\"fixed-case\">ASA<\/jats:styled-content>) of infection with the Y strain of <jats:italic>T.\u00a0cruzi<\/jats:italic>. Seventy\u2010five days after infection, colon samples were collected to quantify inflammatory foci in histological sections and also general (myosin\u2010V<jats:sup>+<\/jats:sup>), nitrergic, and <jats:styled-content style=\"fixed-case\">VIP<\/jats:styled-content>ergic myenteric neurons in whole mounts. Gastrointestinal transit time was also measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Aspirin treatment during the acute phase of infection reduced parasitemia (<jats:italic>P<\/jats:italic>&lt;.05). Aspirin treatment during the acute or chronic phase of the infection reduced the intensity of inflammatory foci in the colon, protected myenteric neurons from cell death and plastic changes, and recovered the gastrointestinal transit of mice infected with <jats:italic>T.\u00a0cruzi<\/jats:italic> (<jats:italic>P<\/jats:italic>&lt;.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion &amp; Inferences<\/jats:title><jats:p>Early and delayed treatment with low\u2010dose <jats:styled-content style=\"fixed-case\">ASA<\/jats:styled-content> can reduce the morphofunctional damage of colonic myenteric neurons caused by murine <jats:italic>T.\u00a0cruzi<\/jats:italic> infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.13102","type":"journal-article","created":{"date-parts":[[2017,5,19]],"date-time":"2017-05-19T10:42:02Z","timestamp":1495190522000},"page":"1-13","source":"Crossref","is-referenced-by-count":18,"title":["Myenteric neuroprotective role of aspirin in acute and chronic experimental infections with <i>Trypanosoma cruzi<\/i>"],"prefix":"10.1111","volume":"29","author":[{"given":"J. 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M.","family":"Carlos","sequence":"additional","affiliation":[{"name":"Department of Histology State University of Londrina  Londrina Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Gouveia","sequence":"additional","affiliation":[{"name":"Department of Histology State University of Londrina  Londrina Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. F. C.","family":"Luchetti","sequence":"additional","affiliation":[{"name":"Department of Pathological Science State University of Londrina  Londrina Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. M.","family":"Moreira","sequence":"additional","affiliation":[{"name":"Center for Education, Letters and Health State University of Western Paran\u00e1  Foz do Igua\u00e7u Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. L.","family":"Massocatto","sequence":"additional","affiliation":[{"name":"Department of Morphological Science State University of Maring\u00e1  Maring\u00e1 Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. M.","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Department of Basic Health Science State University of Maring\u00e1  Maring\u00e1 Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. M. G.","family":"Sant\u00b4Ana","sequence":"additional","affiliation":[{"name":"Department of Morphological Science State University of Maring\u00e1  Maring\u00e1 Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. 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