{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:45:23Z","timestamp":1790739923509,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,6,28]],"date-time":"2017-06-28T00:00:00Z","timestamp":1498608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81270720"],"award-info":[{"award-number":["81270720"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81471487"],"award-info":[{"award-number":["81471487"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2017,12]]},"abstract":"Abstract<\/jats:title>Aim<\/jats:title>The goal of this study was to investigate the expression of fibronectin (FN<\/jats:styled-content>) and the correlated abundance of neuroligins (NL<\/jats:styled-content>s) in the enteric nervous system (ENS<\/jats:styled-content>) and to find a novel diagnostic marker in the serum of Hirschsprung disease (HSCR<\/jats:styled-content>) patients.<\/jats:p><\/jats:sec>Methods<\/jats:title>The expression levels of FN<\/jats:styled-content>, neuroligin\u20101 and neuroligin\u20102 were detected in 114 children with or without HSCR<\/jats:styled-content>. The expression and localization of the NL<\/jats:styled-content>s and FN<\/jats:styled-content> were assessed morphologically by immunohistochemical staining. Western blot analysis and real\u2010time fluorescence quantitative PCR<\/jats:styled-content> (qPCR<\/jats:styled-content>) were performed to examine the correlated expression of the NL<\/jats:styled-content>s and FN<\/jats:styled-content> in aganglionic, transitional, and normal ganglionic colon tissues. An enzyme\u2010linked immunosorbent assay (ELISA<\/jats:styled-content>) was performed to evaluate and compare serum FN<\/jats:styled-content> levels between HSCR<\/jats:styled-content> and non\u2010HSCR<\/jats:styled-content>and between long\u2010type HSCR<\/jats:styled-content> and short\u2010type HSCR<\/jats:styled-content>.<\/jats:p><\/jats:sec>Results<\/jats:title>These studies showed that both neuroligin\u20101 and neuroligin\u20102 were expressed at low levels in aganglionic segments and at intermediate levels in transitional segments compared to their high level of expression in normal tissue. In contrast, FN<\/jats:styled-content> expression was negatively correlated, with expression in these three samples transitioning from highest to lowest. The serum FN<\/jats:styled-content> level was higher in HSCR<\/jats:styled-content> than in non\u2010HSCR<\/jats:styled-content>, but no significant difference between short\u2010type HSCR<\/jats:styled-content> and long\u2010type HSCR<\/jats:styled-content> was observed.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>FN<\/jats:styled-content> affects the expression of both neuroligin\u20101 and neuroligin\u20102 in HSCR<\/jats:styled-content>, which may lead to the hypoplasia of ganglion cells in the ENS<\/jats:styled-content>. This correlation may play a key role in the pathogenesis, diagnosis, or classification of HSCR<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.13134","type":"journal-article","created":{"date-parts":[[2017,6,28]],"date-time":"2017-06-28T08:02:44Z","timestamp":1498636964000},"source":"Crossref","is-referenced-by-count":9,"title":["Down\u2010regulation of fibronectin and the correlated expression of neuroligin in hirschsprung disease"],"prefix":"10.1111","volume":"29","author":[{"given":"Y.","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Pediatric Surgery Qilu Hospital Shandong University Jinan Shandong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Qilu Hospital Shandong University Jinan Shandong Province 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