{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T01:56:13Z","timestamp":1782266173034,"version":"3.54.5"},"reference-count":34,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,6,28]],"date-time":"2017-06-28T00:00:00Z","timestamp":1498608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["25460911"],"award-info":[{"award-number":["25460911"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["15K08929"],"award-info":[{"award-number":["15K08929"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24248050"],"award-info":[{"award-number":["24248050"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["25221205"],"award-info":[{"award-number":["25221205"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004330","name":"Smoking Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004330","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2017,12]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>We previously reported that Daikenchuto (DKT<\/jats:styled-content>), a gastrointestinal prokinetic Japanese herbal (Kampo) medicine used for the treatment of postoperative ileus (POI<\/jats:styled-content>), has characteristic potent anti\u2010inflammatory activity. This effect may be partly mediated by the activation of \u03b17 nicotinic acetylcholine receptor (nAChR<\/jats:styled-content>). In this study, we identified the specific herbs in DKT<\/jats:styled-content> that induce anti\u2010inflammatory action.<\/jats:p><\/jats:sec>Methods<\/jats:title>The herbal components of DKT<\/jats:styled-content> were individually administered orally to each mouse four times before and after intestinal manipulation (IM<\/jats:styled-content>) was carried out on the distal ileum. The anti\u2010inflammatory activity of each crude drug was subsequently evaluated using immunohistochemical analyses of relevant molecules.<\/jats:p><\/jats:sec>Key Results<\/jats:title>Treatment with Zingiberis Siccatum Rhizoma (ZSR<\/jats:styled-content>) but not the other components inhibited the infiltration of cluster of differentiation 68 (CD<\/jats:styled-content>68)\u2010positive macrophages as effectively as DKT<\/jats:styled-content> treatment. Selective \u03b17nAC<\/jats:styled-content>hR antagonists, such as methyllycaconitine citrate, or transient receptor potential ankyrin 1 (TRPA<\/jats:styled-content>1) antagonists, such as HC<\/jats:styled-content>\u2010030031, significantly inhibited the amelioration of macrophage infiltration by ZSR<\/jats:styled-content>. The inhibition of macrophage infiltration by ZSR<\/jats:styled-content> was abolished in both \u03b17nAC<\/jats:styled-content>hR and 5\u2010hydroxytryptamine 4 receptor (5\u2010HT<\/jats:styled-content>4<\/jats:sub>R) knockout mice.<\/jats:p><\/jats:sec>Conclusions & Inferences<\/jats:title>Daikenchuto\u2010induced anti\u2010inflammatory activity, which was mediated by inhibiting macrophage infiltration in POI<\/jats:styled-content>, is dependent on the effects of ZSR<\/jats:styled-content>. Zingiberis Siccatum Rhizoma activates TRPA<\/jats:styled-content>1 channels possibly in enterochromaffin (EC<\/jats:styled-content>) cells to release 5\u2010HT<\/jats:styled-content>, which stimulates 5\u2010HT<\/jats:styled-content>4<\/jats:sub>R in the myenteric plexus neurons to release AC<\/jats:styled-content>h, which in turn activates \u03b17nAC<\/jats:styled-content>hR on macrophages to inhibit inflammation in POI<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.13139","type":"journal-article","created":{"date-parts":[[2017,6,28]],"date-time":"2017-06-28T08:22:39Z","timestamp":1498638159000},"source":"Crossref","is-referenced-by-count":17,"title":["Zingiberis Siccatum Rhizoma, the active component of the Kampo formula Daikenchuto, induces anti\u2010inflammatory actions through \u03b17 nicotinic acetylcholine receptor activation"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0454-7647","authenticated-orcid":false,"given":"M.","family":"Endo","sequence":"first","affiliation":[{"name":"Department of Clinical Research Oriental Medicine Research Center Kitasato University Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Hori","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pharmacology Graduate School of Agriculture and Life Sciences The University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Mihara","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pharmacology Graduate School of Agriculture and Life Sciences The University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Ozaki","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pharmacology Graduate 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