{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T17:43:46Z","timestamp":1783619026062,"version":"3.55.0"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,8,7]],"date-time":"2017-08-07T00:00:00Z","timestamp":1502064000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Fr 1725\/1\u20105"],"award-info":[{"award-number":["Fr 1725\/1\u20105"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Fr 1725\/5\u20101"],"award-info":[{"award-number":["Fr 1725\/5\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2018,3]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>Gastrointestinal (GI<\/jats:styled-content>) motility originates from coordinated movements of circular (CM<\/jats:styled-content>) and longitudinal (LM<\/jats:styled-content>) smooth muscle. How the two muscle layers react individually to nitrergic input and how they integrate nitrergic signaling is not thoroughly understood.<\/jats:p><\/jats:sec>Methods<\/jats:title>We used immunohistochemistry to unveil expression of NO<\/jats:styled-content>\u2010sensitive guanylyl cyclase (NO<\/jats:styled-content>\u2010GC<\/jats:styled-content>) in the ileum. For functional analyses, we measured tone of ileal CM<\/jats:styled-content> and spontaneous contractions in both ileal muscle layers from mice lacking NO<\/jats:styled-content>\u2010GC<\/jats:styled-content> globally (GCKO<\/jats:styled-content>) and specifically in smooth muscle cells (SMC<\/jats:styled-content>\u2010GCKO<\/jats:styled-content>).<\/jats:p><\/jats:sec>Key Results<\/jats:title>In contrast to other parts of the GI<\/jats:styled-content> tract, NO<\/jats:styled-content>\u2010GC<\/jats:styled-content> was not expressed in ckit\u2010positive cells in ileum. NO<\/jats:styled-content>\u2010GC<\/jats:styled-content> expression was intense in platelet\u2010derived growth factor receptor \u03b1\u2010positive cells and in yet unidentified cells of myenteric plexus and serosa. Both CM<\/jats:styled-content> and LM<\/jats:styled-content> developed spontaneous contractile activity; frequency and duration of their spontaneous contractions were identical. The amplitude of spontaneous contractions in CM<\/jats:styled-content> was increased in the absence of NO<\/jats:styled-content>\u2010GC<\/jats:styled-content>. In ileum from control (ctrl) animals, inhibition of NO<\/jats:styled-content>\u2010GC<\/jats:styled-content> increased whereas NO<\/jats:styled-content>\u2010GC<\/jats:styled-content> stimulation decreased tissue tone. In contrast, contractile activity in LM<\/jats:styled-content> was not different between ctrl and knockout strains. Here, NO<\/jats:styled-content> led to suppression of spontaneous contractions of ctrl ileum whereas GCKO<\/jats:styled-content> tissue was unaffected. To our surprise, NO<\/jats:styled-content> suppressed spontaneous contractions in SMC<\/jats:styled-content>\u2010GCKO<\/jats:styled-content> ileum indicating participation of other cell type(s).<\/jats:p><\/jats:sec>Conclusions and Inferences<\/jats:title>NO<\/jats:styled-content>\u2010GC<\/jats:styled-content> in SMC<\/jats:styled-content> is involved in the regulation of tone and amplitude of spontaneous contractions in ileal CM<\/jats:styled-content>. In LM<\/jats:styled-content>, NO<\/jats:styled-content> induces suppression of spontaneous contractions via NO<\/jats:styled-content>\u2010GC<\/jats:styled-content> in a non\u2010SMC<\/jats:styled-content> type.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.13175","type":"journal-article","created":{"date-parts":[[2017,8,7]],"date-time":"2017-08-07T07:06:36Z","timestamp":1502089596000},"source":"Crossref","is-referenced-by-count":8,"title":["Comparison of nitrergic signaling in circular and longitudinal smooth muscle of murine ileum"],"prefix":"10.1111","volume":"30","author":[{"given":"B.","family":"Voussen","sequence":"first","affiliation":[{"name":"Physiologisches Institut Universit\u00e4t W\u00fcrzburg W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Beck","sequence":"additional","affiliation":[{"name":"Physiologisches Institut Universit\u00e4t W\u00fcrzburg W\u00fcrzburg 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