{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T17:18:26Z","timestamp":1790443106904,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,8,24]],"date-time":"2017-08-24T00:00:00Z","timestamp":1503532800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>It remains unclear whether regional variation exists in the human enteric nervous system (<jats:styled-content style=\"fixed-case\">ENS<\/jats:styled-content>) ie, whether intrinsic innervation varies along the gut. Recent classification of gastrointestinal neuropathies has highlighted inadequacies in the quantification of the human <jats:styled-content style=\"fixed-case\">ENS<\/jats:styled-content>. This study used paired wholemounts to accurately quantify and neurochemically code the hindgut myenteric plexus, comparing human distal colon and rectum.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Paired human descending colonic\/rectal specimens were procured from 15 patients undergoing anterior resection. Wholemounts of myenteric plexi were triple\u2010immunostained with anti\u2010Hu\/<jats:styled-content style=\"fixed-case\">NOS<\/jats:styled-content>\/Ch<jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content> antibodies. Images were acquired by motorized epifluorescence microscopy, allowing assessment of ganglionic density\/size, ganglionic area density, and neuronal density. \u2018Stretch\u2010corrected\u2019 values were calculated using stretched\/relaxed tissue dimensions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>Tile\u2010stitching created a collage with average area 99\u00a0300\u00a0000\u00a0\u03bcm<jats:sup>2<\/jats:sup>. Stretch\u2010corrected ganglionic densities were similar (colon: median 510\u00a0ganglia\/100\u00a0mm<jats:sup>2<\/jats:sup> [range 386\u20101170], rectum: 585 [307\u2010923]; <jats:italic>P<\/jats:italic>\u00a0=\u00a0.99), as were average ganglionic sizes (colon: 57\u00a0593\u00a0\u03bcm<jats:sup>2<\/jats:sup> [40\u00a0301\u2010126\u00a0579], rectum: 54\u00a0901 [38\u00a0701\u201090\u00a0211], <jats:italic>P<\/jats:italic>\u00a0=\u00a0.36). Ganglionic area density (colon: 11.92\u00a0mm<jats:sup>2<\/jats:sup> per 100\u00a0mm<jats:sup>2<\/jats:sup> [7.53\u201018.64], rectum: 9.84 [5.80\u201017.19], <jats:italic>P<\/jats:italic>\u00a0=\u00a0.10) and stretch\u2010corrected neuronal densities (colon: 189 neurons\/mm<jats:sup>2<\/jats:sup> [117\u2010388], rectum: 182 [89\u2010361], <jats:italic>P<\/jats:italic>\u00a0=\u00a0.31) were also similar, as were the neurochemical profiles of myenteric ganglia, with comparable proportions of <jats:styled-content style=\"fixed-case\">NOS<\/jats:styled-content>+ and Ch<jats:styled-content style=\"fixed-case\">AT<\/jats:styled-content>+ neurons (<jats:italic>P\u00a0<\/jats:italic>&gt;\u00a0.10).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Inferences<\/jats:title><jats:p>This study has revealed similar neuronal and ganglionic densities and neurochemical profiles in human distal colon and rectum. Further investigation of other components of the <jats:styled-content style=\"fixed-case\">ENS<\/jats:styled-content>, incorporating additional immunohistochemical markers are required to confirm that there is no regional variation in the human hindgut <jats:styled-content style=\"fixed-case\">ENS<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.13193","type":"journal-article","created":{"date-parts":[[2017,8,24]],"date-time":"2017-08-24T09:06:41Z","timestamp":1503565601000},"source":"Crossref","is-referenced-by-count":14,"title":["Quantification and neurochemical coding of the myenteric plexus in humans: No regional variation between the distal colon and rectum"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7614-855X","authenticated-orcid":false,"given":"K.\u2010S.","family":"Ng","sequence":"first","affiliation":[{"name":"Academic Colorectal Unit Sydney Medical School \u2010 Concord University of Sydney  Concord New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. A.","family":"Montes\u2010Adrian","sequence":"additional","affiliation":[{"name":"Academic Colorectal Unit Sydney Medical School \u2010 Concord University of Sydney  Concord New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. A.","family":"Mahns","sequence":"additional","affiliation":[{"name":"Department of Integrative Physiology School of Medicine University of Western Sydney  Campbelltown New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. A.","family":"Gladman","sequence":"additional","affiliation":[{"name":"Academic Colorectal Unit Sydney Medical School \u2010 Concord University of Sydney  Concord New South Wales Australia"},{"name":"Enteric Neuroscience and Gastrointestinal Research Group Adelaide Medical School The University of Adelaide  Adelaide South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,8,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1899.sp000752"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2012.32"},{"issue":"8","key":"e_1_2_9_4_1","first-page":"S129","article-title":"The enteric nervous system","volume":"89","author":"Costa M","year":"1994","journal-title":"Am J Gastroenterol"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1746.17.s1.7.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2009.200444"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2009.184572"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2008.01165.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00021.2017"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0004-282X2003000200011"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12141"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2010.01657.x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-009-0527-y"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12114"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2009.01349.x"},{"key":"e_1_2_9_16_1","first-page":"41","article-title":"Investigation of general and cytoskeletal markers to estimate numbers and proportions of neurons in the human intestine","volume":"21","author":"Ganns D","year":"2006","journal-title":"Histol Histopathol"},{"key":"e_1_2_9_17_1","volume-title":"Meta Imaging Series Metamorph Basic Commands","author":"Molecular Devices Corporation","year":"2006"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-003-0951-7"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2008.01245.x"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1350-1925.2003.00442.x"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2006.00843.x"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-006-9680-5"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-010-0981-9"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-009-0770-5"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2010.01524.x"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.22679"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2009.01445.x"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00384-011-1282-9"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(96)00275-8"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2012.11.012"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mcg.0000173856.84814.37"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e31819b381a"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(97)70011-8"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.51.1.70"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-010-0774-y"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-011-0807-1"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchemneu.2007.03.001"}],"container-title":["Neurogastroenterology &amp; 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