{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:42:38Z","timestamp":1753879358371,"version":"3.41.2"},"reference-count":23,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2017,12,29]],"date-time":"2017-12-29T00:00:00Z","timestamp":1514505600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Vice Chancellor of Research Affairs of Ahvaz Jundishapur University of Medical Sciences","award":["APRC-95-20"],"award-info":[{"award-number":["APRC-95-20"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2018,10]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>The inhibitory effects of H2<\/jats:sub>S on spontaneous contractions of smooth muscles of small, and large intestines well\u2010established but its role in the pathophysiology of diarrhea has not been identified. Therefore, this study evaluated the role of exogenous H2<\/jats:sub>S (NaHS<\/jats:styled-content>) on diabetic\u2010induced diarrhea and determined mRNA<\/jats:styled-content> expression of cystathionine \u03b2\u2010lyase (CSE<\/jats:styled-content>) and cystathionine \u03b3\u2010synthase (CBS<\/jats:styled-content>) in diabetic rats.<\/jats:p><\/jats:sec>Methods<\/jats:title>In order to evaluate antidiarrheal effect of H2<\/jats:sub>S, normal and diabetic rats received NaHS<\/jats:styled-content> and L\u2010Cysteine and the total number of fecal pellets (FP<\/jats:styled-content>) determined. The effect of NaHS<\/jats:styled-content> on intestinal transit ratio (ITR<\/jats:styled-content>) was also evaluated in diabetic rats. The level of mRNA<\/jats:styled-content> expressions of CBS<\/jats:styled-content> and CSE<\/jats:styled-content> determined in smooth muscles of jejunum, ileum, and colon in normal, and diabetic rats. The effect of NaHS<\/jats:styled-content> on frequency and tension of spontaneous contractions of smooth muscle strips of colon, ileum, and jejunum were investigated.<\/jats:p><\/jats:sec>Key Results<\/jats:title>NaHS<\/jats:styled-content> decreased ITR<\/jats:styled-content>, total number of FP<\/jats:styled-content>, frequency and tension of spontaneous contractions of colon, ileum, and jejunum muscle strips in diabetic rats. The level of mRNA<\/jats:styled-content> expression of CSE<\/jats:styled-content> and CBS<\/jats:styled-content> in diabetic rats were lower than in normal rats. NaHS<\/jats:styled-content>, and L\u2010Cysteine decreased the number of FP<\/jats:styled-content> in normal rats.<\/jats:p><\/jats:sec>Conclusions & Inferences<\/jats:title>These findings showed NaHS<\/jats:styled-content> effectively controlled diarrhea in diabetic rats through decreasing the frequency, and tension of spontaneous contraction of smooth muscles of large, and small intestines. The increased frequency and tension of spontaneous contractions of smooth muscles in diabetic rats may be due to down\u2010regulation of H2<\/jats:sub>S biosynthesis enzymes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.13273","type":"journal-article","created":{"date-parts":[[2017,12,29]],"date-time":"2017-12-29T11:09:21Z","timestamp":1514545761000},"source":"Crossref","is-referenced-by-count":4,"title":["Antidiarrheal effect of sodium hydrosulfide in diabetic rats: In vitro and in vivo studies"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0888-9655","authenticated-orcid":false,"given":"M.","family":"Saghazadeh\u2010Dezfuli","sequence":"first","affiliation":[{"name":"Physiology Research Center (PRC) Research Center for Infectious Diseases of Digestive System [Alimentary Tract Research Center] Department of Physiology School of Medicine Ahvaz Jundishapur University of Medical Sciences Ahvaz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6829-8307","authenticated-orcid":false,"given":"H.","family":"Fanaei","sequence":"additional","affiliation":[{"name":"Physiology Research Center (PRC) Department of Physiology School of Medicine Ahvaz Jundishapur University of Medical Sciences Ahvaz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9887-7254","authenticated-orcid":false,"given":"M. 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A.","family":"Mard","sequence":"additional","affiliation":[{"name":"Physiology Research Center (PRC) Research Center for Infectious Diseases of Digestive System [Alimentary Tract Research Center] Department of Physiology School of Medicine Ahvaz Jundishapur University of Medical Sciences Ahvaz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1990.00390140018005"},{"key":"e_1_2_10_3_1","first-page":"340","article-title":"[Chronic diarrhea in the diabetic. 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