{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T11:24:18Z","timestamp":1780485858583,"version":"3.54.1"},"reference-count":25,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,2,14]],"date-time":"2018-02-14T00:00:00Z","timestamp":1518566400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100001003","name":"Boehringer Ingelheim","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001003","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neurogastroenterology Motil"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Stimulant laxatives are widely used to treat constipation. We investigated in human small and large intestinal preparations the effects of <jats:italic>bis<\/jats:italic>\u2010(p\u2010hydroxyphenyl)\u2010pyridyl\u20102\u2010methane (<jats:styled-content style=\"fixed-case\">BHPM<\/jats:styled-content>), the active metabolite of the laxatives bisacodyl and sodium picosulfate on smooth muscle tone and epithelial secretion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Circular and longitudinal muscle tone of small or large intestinal preparations were recorded with isometric force transducers. Epithelial ion flux (<jats:styled-content style=\"fixed-case\">I<jats:sub>SC<\/jats:sub><\/jats:styled-content>) and tissue resistance was measured with Ussing chamber technique after apical and basolateral <jats:styled-content style=\"fixed-case\">BHPM<\/jats:styled-content> application to large intestinal mucosa\/submucosa preparations. Studies were performed in macroscopically normal specimens from 79 patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">BHPM<\/jats:styled-content> concentration\u2010dependently (0.5\u20105\u00a0\u03bcM) increased the tone of circular and longitudinal muscle from small to large intestine. The effect was strongest in large intestinal longitudinal muscle and smallest in small intestinal circular muscle. Increase in muscle tone was prevented by the L\u2010type Ca<jats:sup>++<\/jats:sup> channel blocker nifedipine but insensitive to the nerve blocker tetrodotoxin. Apical or basolateral <jats:styled-content style=\"fixed-case\">BHPM<\/jats:styled-content> concentration\u2010dependently decreased or increased <jats:styled-content style=\"fixed-case\">I<jats:sub>SC<\/jats:sub><\/jats:styled-content><jats:sub>,<\/jats:sub> respectively. The <jats:styled-content style=\"fixed-case\">K<jats:sub>C<\/jats:sub><\/jats:styled-content><jats:sub>a<\/jats:sub>1.1 (<jats:styled-content style=\"fixed-case\">BK<\/jats:styled-content>) channel blocker iberiotoxin reversed apical <jats:styled-content style=\"fixed-case\">I<jats:sub>SC<\/jats:sub><\/jats:styled-content> decrease whereas tetrodotoxin reversed basolateral <jats:styled-content style=\"fixed-case\">I<jats:sub>SC<\/jats:sub><\/jats:styled-content> increase. <jats:styled-content style=\"fixed-case\">BHPM<\/jats:styled-content> had no effect on tissue resistance or nerve\u2010mediated secretory or muscle response with one exception: at the highest concentration basolateral <jats:styled-content style=\"fixed-case\">BHPM<\/jats:styled-content> reduced nerve\u2010mediated secretion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and Interferences<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">BHPM<\/jats:styled-content> enhanced mucosal secretion and muscle contractility. Results suggested that the laxative effect of <jats:styled-content style=\"fixed-case\">BHPM<\/jats:styled-content> was a consequence of the increase in muscle tone as well as an increased K<jats:sup>+<\/jats:sup> secretion when acting luminally and a nerve\u2010driven Cl<jats:sup>\u2212<\/jats:sup> and <jats:styled-content style=\"fixed-case\">HCO<\/jats:styled-content><jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> secretion once acting basolaterally after absorption.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.13311","type":"journal-article","created":{"date-parts":[[2018,2,14]],"date-time":"2018-02-14T05:36:36Z","timestamp":1518586596000},"source":"Crossref","is-referenced-by-count":26,"title":["<i>bis<\/i>\u2010(p\u2010hydroxyphenyl)\u2010pyridyl\u20102\u2010methane (<scp>BHPM<\/scp>)\u2014the active metabolite of the laxatives bisacodyl and sodium picosulfate\u2014enhances contractility and secretion in human intestine in vitro"],"prefix":"10.1111","volume":"30","author":[{"given":"D.","family":"Krueger","sequence":"first","affiliation":[{"name":"Human Biology Technische Universit\u00e4t M\u00fcnchen  Freising Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I. E.","family":"Demir","sequence":"additional","affiliation":[{"name":"Department of Surgery Klinikum Rechts der Isar Technische Universit\u00e4t M\u00fcnchen  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. O.","family":"Ceyhan","sequence":"additional","affiliation":[{"name":"Department of Surgery Klinikum Rechts der Isar Technische Universit\u00e4t M\u00fcnchen  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Zeller","sequence":"additional","affiliation":[{"name":"Surgery Clinic Freising  Freising Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1007-9843","authenticated-orcid":false,"given":"M.","family":"Schemann","sequence":"additional","affiliation":[{"name":"Human Biology Technische Universit\u00e4t M\u00fcnchen  Freising Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,2,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2016-311835"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2011.03.026"},{"key":"e_1_2_9_4_1","first-page":"1796","article-title":"Bis\u2010(p\u2010hydroxyphenyl)\u2010pyridyl\u20102\u2010methane: the common laxative principle of bisacodyl and sodium picosulfate","volume":"25","author":"Jauch R","year":"1975","journal-title":"Arzneimittelforschung"},{"key":"e_1_2_9_5_1","first-page":"570","article-title":"Pharmakokinetik und laxierende Wirkung von Bisacodyl nach Gabe verschiedener Zubereitungsformen","volume":"38","author":"Roth W","year":"1988","journal-title":"Arzneimittelforschung"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000138551"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01468863"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000199105"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01468637"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2004.00535.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12771"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12156"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2008.01242.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP271493"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.080002"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.2133\/dmpk.DMPK-11-RG-007"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118105955"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00341220"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02088131"},{"key":"e_1_2_9_20_1","first-page":"705","article-title":"Mechanism of action of Trolox on duodenal contractility","volume":"64","author":"Fagundes DS","year":"2013","journal-title":"J Physiol Pharmacol"},{"key":"e_1_2_9_21_1","first-page":"479","article-title":"Entacetylierung und Resorption von phenolischen Laxantien","volume":"29","author":"Ferlemann G","year":"1965","journal-title":"Naunyn Schmiedebergs Arch Exp Pathol Pharmakol"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.2220\/biomedres.37.13"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00286.2011"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1159\/000199508"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.12011"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2004.00548.x"}],"container-title":["Neurogastroenterology &amp; Motility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fnmo.13311","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/nmo.13311","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T16:07:12Z","timestamp":1694880432000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/nmo.13311"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2,14]]},"references-count":25,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2018,7]]}},"alternative-id":["10.1111\/nmo.13311"],"URL":"https:\/\/doi.org\/10.1111\/nmo.13311","archive":["Portico"],"relation":{},"ISSN":["1350-1925","1365-2982"],"issn-type":[{"value":"1350-1925","type":"print"},{"value":"1365-2982","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,2,14]]},"article-number":"e13311"}}