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Here, we characterize gastrointestinal (GI<\/jats:styled-content>) dysmotility in the experimental autoimmune encephalomyelitis (EAE<\/jats:styled-content>) mouse model of MS<\/jats:styled-content> and evaluate whether autoantibodies target the enteric nervous system (ENS<\/jats:styled-content>) and cause dysmotility.<\/jats:p><\/jats:sec>Methods<\/jats:title>EAE<\/jats:styled-content> was induced in male SJL<\/jats:styled-content> and B6 mice. GI<\/jats:styled-content> motility was assessed in vivo<\/jats:italic> and ex vivo<\/jats:italic> in wild type (WT<\/jats:styled-content>) and B cell\u2010deficient mice. MS<\/jats:styled-content> and EAE<\/jats:styled-content> serum was used to survey potential targets in the ENS<\/jats:styled-content> and changes in the ENS<\/jats:styled-content> structure were characterized using immunohistochemistry.<\/jats:p><\/jats:sec>Key Results<\/jats:title>EAE<\/jats:styled-content> mice developed accelerated gastric emptying and delayed whole GI<\/jats:styled-content> transit with reduced colonic motility. Fecal water content was reduced, and colonic migrating myoelectrical complexes (CMMC<\/jats:styled-content>) and slow waves were less frequent. Colons from EAE<\/jats:styled-content> mice exhibited decreased GFAP<\/jats:styled-content> levels in glia. Sera from MS<\/jats:styled-content> patients and from EAE<\/jats:styled-content> mice targeted ENS<\/jats:styled-content> neurons and glia. B\u2010cell deficiency in EAE<\/jats:styled-content> protected against colonic dysmotility.<\/jats:p><\/jats:sec>Conclusions & Inferences<\/jats:title>Consistent with symptoms experienced in MS<\/jats:styled-content>, we demonstrate that EAE<\/jats:styled-content> mice widely exhibit features of GI<\/jats:styled-content> dysmotility that persisted in the absence of extrinsic innervation, suggesting direct involvement of ENS<\/jats:styled-content> neurocircuitry. The absence of GI<\/jats:styled-content> dysmotility in B cell\u2010deficient mice with EAE<\/jats:styled-content> together with EAE<\/jats:styled-content> and MS<\/jats:styled-content> serum immunoreactivity against ENS<\/jats:styled-content> targets suggests that MS<\/jats:styled-content> could be classified among other diseases known to induce autoimmune GI<\/jats:styled-content> dysmotility.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.13349","type":"journal-article","created":{"date-parts":[[2018,4,12]],"date-time":"2018-04-12T05:05:33Z","timestamp":1523509533000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":66,"title":["Altered gastrointestinal motility involving autoantibodies in the experimental autoimmune encephalomyelitis model of multiple sclerosis"],"prefix":"10.1111","volume":"30","author":[{"given":"E. 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