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Stomach ulcer, a disorder commonly occurring in humans and animals, is accompanied by inflammatory reaction. Inflammation can cause intramural neurons to change their neurochemical profile. Galanin and its receptors are involved in inflammation of many organs, however, their direct participation in stomach reaction to ulcer is not known. Therefore, the aim of the study was to investigate adaptive changes in the chemical coding of galaninergic intramural neurons and <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression encoding Gal, GalR1, GalR2, GalR3 receptors in the region of the porcine stomach directly adjacent to the ulcer location.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The experiment was performed on 24 pigs, divided into control and experimental groups. In 12 experimental animals, stomach antrum ulcers were experimentally induced by submucosal injection of acetic acid solution. Stomach wall directly adjacent to the ulcer was examined by: (1) double immunohistochemistry\u2014to verify the changes in the number of galaninergic neurons (submucosal, myenteric) and fibers; (2) real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> to verify changes in <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression encoding galanin, GalR1, GalR2, GalR3 receptors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key Results<\/jats:title><jats:p>In the experimental animals, the number of Gal\u2010immunoreactive submucosal perikarya was increased, while the number of galaninergic myenteric neurons and fibers (in all the stomach wall layers) remained unchanged. The expression of <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> encoding all galanin receptors was increased.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions &amp; Interferences<\/jats:title><jats:p>The results obtained unveiled the participation of galanin and galanin receptors in the stomach tissue response to antral ulcerations.<\/jats:p><\/jats:sec>","DOI":"10.1111\/nmo.13360","type":"journal-article","created":{"date-parts":[[2018,5,2]],"date-time":"2018-05-02T09:32:29Z","timestamp":1525253549000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Galaninergic intramural nerve and tissue reaction to antral ulcerations"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1626-218X","authenticated-orcid":false,"given":"M.","family":"Zalecki","sequence":"first","affiliation":[{"name":"Department of Animal Anatomy Faculty of Veterinary Medicine 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